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Polarized-Light Interferometry of Calcium Carbonate Deposition in Moss from a Waterfall on the Niagara Escarpment

Published online by Cambridge University Press:  30 March 2010

Howard J. Swatland
Howard J. Swatland
Affiliation:
University of Guelph, Guelph, Ontario N1G 2W1, Canada
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Abstract

Deposition of calcium carbonate from groundwater was examined on a moss, Didymodon tophaceus, from a Niagara Escarpment waterfall. A spectrophotometer on a polarizing microscope was used for interferometry. A second-order blue interference with an interference minimum around 620 nm was found when moss cell spaces were fully calcified. Filled cell spaces were often surrounded by empty cell spaces. Complete calcification of whole leaflets resulted in progressively higher orders of interference colors and a positive shift in interference minima. Calcified leaflets finally became cemented together, but each retained a weak extinction when rotated. Small calcareous spherulites (mean diameter 15.7 ± 2.1 μm) were found between leaflets. Spherulites exhibited first-order white interference with a Maltese cross that rotated when the polarizer and analyzer were rotated in tandem. A Nikitin-Berek compensator was tilted at 5.5° to give an interference minimum at 600 nm in the optical axis. Quadrants of spherulites with radii more or less in line with the tilting axis of the compensator had lower (P < 0.001) interference minima (535 ± 27 nm) than quadrants with radii more or less perpendicular to the compensator (659 ± 15 nm), thus indicating a radial internal structure. Spherulites were tentatively identified as vaterite.

Keywords

polarized lightinterferometrytravertineDidymodon tophaceusNiagara Escarpmentvaterite
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 3 , June 2010 , pp. 306 - 312
Copyright
Copyright © Microscopy Society of America 2010

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References

REFERENCES

Bowden, W.B., Glime, J.M. & Riis, T. (2006). Macrophytes and bryophytes. In Methods in Stream Ecology, Hauer, F.R. & Lamberti, G.A. (Eds.), pp. 381–414. Burlington, MA: Academic Press.Google Scholar
Braissant, O., Cailleau, G., Dupraz, C. & Verrecchia, E.P. (2003). Bacterially induced mineralization of calcium carbonate in terrestrial environments: The role of exopolysaccharides and amino acids. J Sediment Res 73, 485–490.CrossRefGoogle Scholar
Breckinridge, J.B. (1971). Polarization properties of a grating monochromator. Appl Optics 10, 286–294.CrossRefGoogle Scholar
Carpenter, W.B. (1868). The Microscope and Its Revelations, 4th ed., pp. 398–400. London: John Churchill.Google Scholar
Chen, J., Zhang, D.D., Wang, S., Xiao, T. & Huang, R. (2004). Factors controlling tufa deposition in natural waters at waterfall sites. Sediment Geol 166, 353–366.CrossRefGoogle Scholar
Conard, H.S. & Redfearn, P.L. (1979). How to Know the Mosses and Liverworts, 2nd ed., p. 95. Dubuque, IA: Brown.Google Scholar
Dickson, J.A.D. (1978). Length-slow and length-fast calcite: A tale of two elongations. Geology 6, 560–561.2.0.CO;2>CrossRefGoogle Scholar
Emig, W.H. (1917). Mosses as rock builders. Oklahoma Acad Sci 1, 38–40.Google Scholar
Eyles, N. (2002). Ontario Rocks, pp. 135–141. Markham, ON: Fitzhenry & Whiteside.Google Scholar
Frey-Wyssling, A. (1969). On the molecular structure of starch granules. Am J Botany 56, 696–701.CrossRefGoogle Scholar
Hargis, P.J., Sobering, T.J., Tisone, G.C. & Wagner, J.S. (1995). Ultraviolet fluorescence detection and identification of protein, DNA, and bacteria. Proc SPIE 2366, 147–153.CrossRefGoogle Scholar
Huxley, A. (1980). Reflections on Muscle, pp. 1–25. Princeton, NJ: Princeton University Press.Google ScholarPubMed
James, J. (1976). Light Microscopic Techniques in Biology and Medicine, pp. 196–205. Leiden, Netherlands: Martinus Nijhoff Medical Division.CrossRefGoogle Scholar
Kerr, P. (1977). Optical Mineralogy. New York: McGraw-Hill.Google Scholar
McConnell, J.D.C. (1959). Vaterite from Ballycraigy, Larne, Northern Ireland. Mineral Mag 32, 535–544.Google Scholar
Merz-Preiss, M. & Riding, R. (1999). Cyanobacterial tufa calcification in two freshwater streams: Ambient environment, chemical thresholds and biological processes. Sediment Geol 126, 103–124.CrossRefGoogle Scholar
Moore, C.H. (1989). Carbonate Diagenesis and Porosity. Developments in Sedimentology 46, p. 5. Amsterdam: Elsevier.Google Scholar
Oster, G. (1955). Birefringence and dichroism. In Physical Techniques in Biological Research, Oster, G. & Pollister, A.W. (Eds.), 1, pp. 439–460. New York: Academic Press.Google Scholar
Pentecost, A. (1998). The significance of calcite (travertine) formation by algae in a moss-dominated travertine from Matlock Bath, England. Archiv Hydrobiol 143, 487–509.CrossRefGoogle Scholar
Pentecost, A. (2005). Travertine. Berlin: Springer-Verlag.Google Scholar
Pentecost, A. & Coletta, P. (2007). The role of photosynthesis and CO2 evasion in travertine formation; a quantitative investigation at an important travertine-depositing hot spring, Le Zitelle, Lazio, Italy. J Geol Soc Lond 164, 843–853.CrossRefGoogle Scholar
Riding, R. (2000). Microbial carbonates: The geological record of calcified bacterial-algal mats and biofilms. Sedimentology 47(S1), 179–214.CrossRefGoogle Scholar
Rodie, A. & Post, R. (2009). Niagara Escarpment Baseflow Study, p. 59. Utopia, ON: Nottawasaga Valley Conservation Authority.Google Scholar
Rogerson, M., Pedley, H.M., Wadhawan, J.D. & Middleton, R. (2008). New insights into biological influence on the geochemistry of freshwater carbonate deposits. Geochim Cosmochim Acta 72, 4976–4987.CrossRefGoogle Scholar
Ruch, F. (1966). Birefringence and dichroism of cells and tissues. In Physical Techniques in Biological Research, Oster, G. & Pollister, A.W. (Eds.), 2nd ed., 3, part A, pp. 57–86. New York: Academic Press.Google Scholar
Sato, M. & Matsuda, S. (1969). Structure of vaterite and infrared spectra. Z Kristallogr 129, 405–410.CrossRefGoogle Scholar
Steel, R.G.D. & Torrie, J.H. (1980). Principles and Procedures of Statistics. A Biometrical Approach, 2nd ed., pp. 67–83. New York: McGraw-Hill.Google Scholar
Swatland, H.J. (1998). Computer Operation for Microscope Photometry. Boca Raton, FL: CRC Press.Google Scholar
Swatland, H.J. (2009). Effect of granule size on the interference colors of starch in polarized light. Biotechnic & Histochemistry 84, 329–336.CrossRefGoogle ScholarPubMed
Swatland, H.J. & Barbut, S. (1999). Sodium chloride levels in comminuted chicken muscle in relation to processing characteristics and Fresnel reflectance detected with a polarimetric probe. Meat Sci 51, 377–381.CrossRefGoogle ScholarPubMed