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Novel Non-Injection Approaches for Revealing and for Microscopic Investigation of the Microcirculatory Bed in Different Organs and Tissues

Published online by Cambridge University Press:  02 July 2020

N. Chilingaryan ,
A. Chilingaryan  and
M. Chilingaryan
N. Chilingaryan
Affiliation:
Department of Neurology, # 5637, LAC-USC Medical Center, 1200 N. State St., Los AngelesCA90033.
A. Chilingaryan
Affiliation:
Laboratory of Histochemistry and Neuromorphology, Institute of Physiology, Armenian Academy of Sciences, Yerevan, Armenia375028.
M. Chilingaryan
Affiliation:
Laboratory of Histochemistry and Neuromorphology, Institute of Physiology, Armenian Academy of Sciences, Yerevan, Armenia375028.
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Extract

Certain disadvantages of traditional injection methods and difficulties arising during investigation of experimental and pathological material, limit the possibilities for extensive study of the microcirculatory bed (MCB). This has prompted the authors to develop new non-injection methods for revealing the MCB on histological sections by means of direct staining of the structural elements of the vessel wall. These methods are based on selective precipitation of the extra- or intracellular orthophosphates with ions of Zn, Cd, Co, Ca, Sr, and Pb in the vascular endothelium and smooth muscle cells. These methods (which we have called “histoangiological”) provide clear-cut contrast dyeing of the MCB in different organs and tissues as well as tumors in rat, cat,pig, dog, and in humans.

In these methods, the reaction end product is lead sulfide which provides a high degree of contrast. Vessels and capillaries are detected due to precipitation of the deposit in the vascular endothelium.

Type
From Scanning Probe Microscopy to High Resolution Ultrasound: New Versions of the Vasculature
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 327 - 328
Copyright
Copyright © Microscopy Society of America 1997

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References

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