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Nitric Oxide Synthase in Diabetic Retinopathy in the BBZ/WOR Rat: An Immunocytochemical Study

Published online by Cambridge University Press:  02 July 2020

E. Ann Ellis ,
Maria B. Grant  and
Dennis L. Guberski
E. Ann Ellis
Affiliation:
Department of Medicine, College of Medicine, University of Florida, Gainesville, FL32610
Maria B. Grant
Affiliation:
Department of Medicine, College of Medicine, University of Florida, Gainesville, FL32610
Dennis L. Guberski
Affiliation:
Department of Pathology, University of Massachusetts School of Medicine, Worcester, MA01655
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Extract

Nitric oxide synthase (NOS) catalyzes the conversion of L-arginine to citrulline and the highly reactive free radical species, NO. Two major constitutive isoforms, neuronal and endothelial NOS, have been ented in the retina. These constitutive isoforms produce small amounts of NO while the inducible isoform, iNOS, produces high levels of NO. Excess production of NO by iNOS has beem implicated in the complications of diabetes. Since NO is a highly labile molecule, its potential localization is best achieved by immunocytochemical studies of NOS.

Semiquantitative colloidal gold labeled immunocytochemical studies of endothelial NOS (eNOS) and iNOS were done on sections of neural retina in eyes of obese, noninsulin dependent diabetic BBZ/Wor rats with diabetes of five months to twelve months duration. Age matched, nondiabetic control retinas were from eyes from BBDR/Wor rats.

Levels of iNOS were elevated in diabetic (33.9 ± 10.0 gold particles) as compared to age matched nondiabetic control (3.5 ± 2.8 gold particles) retinas.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1074 - 1075
Copyright
Copyright © Microscopy Society of America

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References

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