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Micromorphology of Epicuticular Waxes and Epistomatal Chambers of Pine Species by Electron Microscopy and White Light Scanning Interferometry

Published online by Cambridge University Press:  19 November 2010

Ki Woo Kim ,
In Jung Lee ,
Chang Soo Kim ,
Don Koo Lee  and
Eun Woo Park
Ki Woo Kim*
Affiliation:
National Instrumentation Center for Environmental Management, Seoul National University, Seoul 151-921, Korea
In Jung Lee
Affiliation:
Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea
Chang Soo Kim
Affiliation:
Department of Forest Genetic Resources, Korea Forest Research Institute, Suwon 441-350, Korea
Don Koo Lee
Affiliation:
Department of Forest Sciences, Seoul National University, Seoul 151-921, Korea
Eun Woo Park*
Affiliation:
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea
*
Corresponding author. E-mail: [email protected]
Corresponding author. E-mail: [email protected]
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Abstract

High-resolution imaging and quantitative surface analysis of epicuticular waxes and epistomatal chambers of pine species were performed by field emission scanning electron microscopy and white light scanning interferometry. Both juvenile and adult needles were collected from the two-year-old seedlings of Pinus rigida and Pinus densiflora and subjected to surface observations. Epicuticular wax structures developed on the cuticle layer as well as in the epistomatal chambers and appeared to occlude the cavities in the two pine species. The stomata of P. densiflora were characterized by more distinctly raised rings around openings than P. rigida. The most common epicuticular wax structures of the two pine species included tubules with terminal openings and coiled rodlets. Wax platelets were deposited on epistomatal chambers. Either rodlets or tubules seemed to be longer and thicker in P. rigida than those in P. densiflora. White light scanning interferometry revealed quantitative surface profiles, demonstrating more ridged (ca. 4 μm high) stomatal apertures and nearly twofold deeper (ca. 20 μm deep) epistomatal chambers of P. densiflora than those of P. rigida. These results suggest that white light scanning interferometry can be applied to unravel the quantitative surface features of epicuticular sculptures on plant leaves.

Keywords

interferometryPinus densifloraPinus rigidaSEMstomatawax
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 1 , February 2011 , pp. 118 - 124
Copyright
Copyright © Microscopy Society of America 2011

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