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Microcontact Printed Substrates for Growing Central Nervous System Neurons and Glia

Published online by Cambridge University Press:  02 July 2020

H. G. Craighead ,
M. Isaacson ,
P. St. John ,
R. Davis ,
G. Banker ,
T. Esch ,
L. Kam ,
W. Shain  and
J. N. Turner
H. G. Craighead
Affiliation:
Applied and Engineering Physics, Cornell University, Ithaca, NY14853
M. Isaacson
Affiliation:
Applied and Engineering Physics, Cornell University, Ithaca, NY14853
P. St. John
Affiliation:
Applied and Engineering Physics, Cornell University, Ithaca, NY14853
R. Davis
Affiliation:
Applied and Engineering Physics, Cornell University, Ithaca, NY14853
G. Banker
Affiliation:
Department of Neuroscience, University of Virginia, Charlottsville, VA22908
T. Esch
Affiliation:
Department of Neuroscience, University of Virginia, Charlottsville, VA22908
L. Kam
Affiliation:
Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY12180
W. Shain
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY12201
J. N. Turner
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY12201
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Extract

The application of nano- and microfabrication to biology is an emerging area linking engineering and biology. We are using microfabrication techniques to make patterns of specific bioactive molecules on glass and silicon surfaces to study central nervous system neurons and glia. Cell adhesion and tissue reaction to implanted silicon based electronic prosthetic devices are being studied, and the methods expanded to interface neurons with integrated circuits to study their electrical properties. The number, type and distribution of cells adhering to particular portions of a substrate can be influenced by patterning amine groups, proteins or polypeptides. We are using microcontact printing as an alternative to lithography for chemical patterning.

Primary cultures of rat cortical astrocytes and hippocampal neurons, and continuous cultures of transformed astrocytes were prepared on microcontact printed patterns produced by an elastomer stamp made from a silicon master. Fig. 1 shows two such patterns prepared by depositing self-assembled monolayers of N1[3-(Trimethoxylsily)propyl] diethylenetriamine (DETA) and octadecyltrichlorosilane (OTS).

Type
Shared Resources: Access to Critical Instrumentation
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 287 - 288
Copyright
Copyright © Microscopy Society of America 1997

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References

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