Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-06T04:02:59.611Z Has data issue: false hasContentIssue false
  • English
  • Français

Histological and Stereological Characterization of Brown Trout (Salmo trutta f. fario) Trunk Kidney

Published online by Cambridge University Press:  11 October 2010

Albina D. Resende ,
Alexandre Lobo-da-Cunha ,
Fernanda Malhão ,
Filipa Franquinho ,
Rogério A.F. Monteiro  and
Eduardo Rocha
Albina D. Resende*
Affiliation:
Laboratory of Cellular, Molecular and Analytical Studies, Interdisciplinary Centre for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory, 4050-123, Porto, Portugal Department of Pathological Anatomy, North Polytechnic Health Institute (IPSN), CESPU, 4585-116, Gandra, Portugal
Alexandre Lobo-da-Cunha
Affiliation:
Laboratory of Cellular, Molecular and Analytical Studies, Interdisciplinary Centre for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory, 4050-123, Porto, Portugal Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4099-003, Porto, Portugal
Fernanda Malhão
Affiliation:
Laboratory of Cellular, Molecular and Analytical Studies, Interdisciplinary Centre for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory, 4050-123, Porto, Portugal Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4099-003, Porto, Portugal
Filipa Franquinho
Affiliation:
Department of Pathological Anatomy, North Polytechnic Health Institute (IPSN), CESPU, 4585-116, Gandra, Portugal
Rogério A.F. Monteiro
Affiliation:
Laboratory of Cellular, Molecular and Analytical Studies, Interdisciplinary Centre for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory, 4050-123, Porto, Portugal Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4099-003, Porto, Portugal
Eduardo Rocha
Affiliation:
Laboratory of Cellular, Molecular and Analytical Studies, Interdisciplinary Centre for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory, 4050-123, Porto, Portugal Department of Microscopy, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4099-003, Porto, Portugal
*
Corresponding author. E-mail: [email protected]
Get access

Abstract

The large variability in kidney morphology among fish makes it difficult to build a “universal” model on its function and structure. Therefore, a morphological study of brown trout trunk kidney was performed, considering potential seasonal and sex effects. Three-year-old specimens of both sexes were collected at four stages of their reproductive cycle. Kidney was processed for light and electron microscopy. The relative volumes of renal components, such as renal corpuscles and different nephron tubules, were estimated by stereological methods. Qualitatively, the general nephron structure of brown trout was similar to that described for other glomerular teleost species. Quantitatively, however, differences in the relative volume of some renal components were detected between sexes and among seasons. Particularly, highest values of vacuolized tubules and new growing tubules were observed after spawning, being more relevant in females. Despite seasonal changes, more linear correlations were found between those parameters and the reno-somatic index than the gonado-somatic index. Thus, we verified that some brown trout renal components undergo sex dependent seasonal variations, suggesting a morphological adaptation of the components to accomplish physiological needs. These findings constitute a baseline for launching studies to know which factors govern the morphological variations and their functional consequences.

Keywords

teleostrenal tubulenephronstereology
Type
Special Section from Portugal Meeting
Information
Microscopy and Microanalysis , Volume 16 , Issue 6 , December 2010 , pp. 677 - 687
Copyright
Copyright © Microscopy Society of America 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, B.G. & Loewen, R.D. (1975). Renal morphology of freshwater trout. Am J Anat 143(1), 93–113.CrossRefGoogle ScholarPubMed
Brown, J.A., Oliver, J.A., Herderson, I.W. & Jackon, B.A. (1980). Angiotensin and single nephron glomerular function in the trout Salmo gairdneri. Am J Physiol 239, R509–R514.Google ScholarPubMed
Elger, M., Hentschel, H., Dawson, M. & Renfro, J.L. (2000). Urinary Tract. London: Academic Press.Google Scholar
Hentschel, H. & Elger, M. (1987). The distal nephron in the kidney of fishes—Introduction. Adv Anat Embryol Cell Biol 108, 1–146.CrossRefGoogle Scholar
Hibiya, T. (1982). An Atlas of Fish Histology—Normal and Pathological Features. New York: Kodansha Ltd. Gustav Fischer Verlag.Google Scholar
Kamunde, C.N., Kisia, S.M. & Mwangangi, J.M. (1997). Morphometry of the kidneys of fresh water percoid and ostariophysian teleosts. Acta Biol Hung 48(1), 29–41.CrossRefGoogle ScholarPubMed
Krohmer, R.W., Martinez, D. & Mason, R.T. (2004). Development of the renal sexual segment in immature snakes: Effect of sex steroid hormones. Comp Biochem Physiol A 139(1), 55–64.CrossRefGoogle ScholarPubMed
Larsen, B.K. & Perkins, E.J. Jr. (2001). Target Organ Toxicity in the Kidney. Boca Raton, FL: CRC Press.Google Scholar
Mulroney, S.E., Woda, C., Johnson, M. & Pesce, C. (1999). Gender differences in renal growth and function after uninephrectomy in adult rats. Kidney Int 56(3), 944–953.CrossRefGoogle ScholarPubMed
Oudar, O., Elger, M., Bankir, L., Ganten, D., Ganten, U. & Kriz, W. (1991). Differences in rat-kidney morphology between males, females and testosterone-treated females. Renal Physiol Biochem 14(3), 92–102.Google ScholarPubMed
Oulmi, Y. & Braunbeck, T. (1996). Toxicity of 4-chloroaniline in early life-stages of zebrafish (Brachydanio rerio). 1. Cytopathology of liver and kidney after microinjection. Arch Environ Contam Toxicol 30(3), 390–402.CrossRefGoogle Scholar
Oulmi, Y., Negele, R.D. & Braunbeck, T. (1995a). Cytopathology of liver and kidney in rainbow-trout Oncorhynchus mykiss after long-term exposure to sublethal concentrations of linuron. Dis Aquat Org 21(1), 35–52.CrossRefGoogle Scholar
Oulmi, Y., Negele, R.D. & Braunbeck, T. (1995b). Segment specificity of the cytological response in rainbow-trout (Oncorhynchus mykiss) renal tubules following prolonged exposure to sublethal concentrations of atrazine. Ecotoxicol Environ Safety 32(1), 39–50.CrossRefGoogle ScholarPubMed
Rangsayatorn, N., Kruatrachue, M., Pokethitiyook, P., Upatham, E.S., Lanza, G.R. & Singhakaew, S. (2004). Ultrastructural changes in various organs of the fish Puntius gonionotus fed cadmium-enriched cyanobacteria. Environ Toxicol 19(6), 585–593.CrossRefGoogle ScholarPubMed
Reimschuessel, R. (2001). A fish model of renal regeneration and development. ILAR J 42(4), 285–291.CrossRefGoogle ScholarPubMed
Resende, A.D. (2008). Seasonal and toxicological study of brown trout kidney and liver peroxisomes. PhD Thesis. Porto, Portugal: University of Porto.Google Scholar
Rojik, I., Nemcsok, J. & Boross, L. (1983). Morphological and biochemical-studies on liver, kidney and gill of fishes affected by pesticides. Acta Biol Hung 34(1), 81–92.Google ScholarPubMed
Sangiao-Alvarellos, S., Polakof, S., Arjona, F.J., García-López, A., del Río, M.P.M., Martínez-Rodríguez, G., Míguez, J.M., Mancera, J.M. & Soengas, J.L. (2006). Influence of testosterone administration on osmoregulation and energy metabolism of gilthead sea bream Sparus auratus. Gen Comp Endocrinol 149, 30–41.CrossRefGoogle ScholarPubMed
Triebskorn, R., Casper, H., Heyd, A., Eikemper, R., Kohler, H.R. & Schwaiger, J. (2004). Toxic effects of the non-steroidal anti-inflammatory drug diclofenac. Part II. Cytological effects in liver, kidney, gills and intestine of rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 68(2), 151–166.CrossRefGoogle ScholarPubMed
Varsamos, S., Nebel, C. & Charmantier, G. (2005). Ontogeny of osmoregulation in postembryonic fish: A review. Comp Biochem Physiol A 141(4), 401–429.CrossRefGoogle ScholarPubMed
Velmurugan, B., Selvanayagam, M., Cengiz, E.I. & Unlu, E. (2007a). The effects of monocrotophos to different tissues of freshwater fish Cirrhinus mrigala. Bull Environ Contam Toxicol 78(6), 450–454.CrossRefGoogle ScholarPubMed
Velmurugan, B., Selvanayagam, M., Cengiz, E.P. & Unlu, E. (2007b). Histopathology of lambda-cyhalothrin on tissues (gill, kidney, liver and intestine) of Cirrhinus mrigala. Environ Toxicol Pharmacol 24(3), 286–291.CrossRefGoogle ScholarPubMed
Vijayan, M.M., Takemura, A. & Mommsen, T.P. (2001). Estradiol impairs hyposmoregulatory capacity in the euryhaline tilapia, Oreochromis mossambicus. Am J Physiol Regulatory Integrative Comp Physiol 281, R1161–R1168.CrossRefGoogle ScholarPubMed
Watanabe, N., Kato, M., Suzuki, N., Inoue, C., Fedorova, S., Hashimoto, H., Maruyama, S., Matsuo, S. & Wakamatsu, Y. (2009). Kidney regeneration through nephron neogenesis in medaka. Develop Growth Differ 51, 135–143.CrossRefGoogle ScholarPubMed
Weber, L.P., Hill, R.L. & Janz, D.M. (2003). Developmental estrogenic exposure in zebrafish (Danio rerio): II. Histological evaluation of gametogenesis and organ toxicity. Aquat Toxicol 63(4), 431–446.CrossRefGoogle ScholarPubMed
Weibel, E.R. (1979). Stereological Methods. Vol. I. Practical Methods for Biological Morphometry. London: Academic Press.Google Scholar
Zhang, Y.M., Huang, D.J., Wang, Y.Q., Liu, J.H., Yu, R.L. & Long, J. (2005). Heavy metal accumulation and tissue damage in goldfish Carassius auratus. Bull Environ Contam Toxicol 75(6), 1191–1199.CrossRefGoogle ScholarPubMed