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Galectin-3 Expression in Pancreatic Cell Lines Under Distinct Autophagy-Inducing Stimulus

Published online by Cambridge University Press:  27 October 2020

Antônio Felix da Silva Filho ,
Lizandra Maia de Sousa Open the ORCID record for Lizandra Maia de Sousa [Opens in a new window] ,
Silvio Roberto Consonni ,
Maira Galdino da Rocha Pitta ,
Hernandes Faustino Carvalho  and
Moacyr Jesus Barreto de Melo Rêgo Open the ORCID record for Moacyr Jesus Barreto de Melo Rêgo [Opens in a new window]
Antônio Felix da Silva Filho
Affiliation:
Immunomodulation and New Therapy Approaches Laboratory (LINAT), Biochemistry Department, Federal University of Pernambuco (UFPE), Cidade Universitária, Recife, Pernambuco 50670-901, Brazil
Lizandra Maia de Sousa
Affiliation:
Laboratory of Cytochemistry and Immunocytochemistry, Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, São Paulo 13083-970, Brazil
Silvio Roberto Consonni
Affiliation:
Laboratory of Cytochemistry and Immunocytochemistry, Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, São Paulo 13083-970, Brazil
Maira Galdino da Rocha Pitta
Affiliation:
Immunomodulation and New Therapy Approaches Laboratory (LINAT), Biochemistry Department, Federal University of Pernambuco (UFPE), Cidade Universitária, Recife, Pernambuco 50670-901, Brazil
Hernandes Faustino Carvalho
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Campinas, São Paulo 13083-970, Brazil
Moacyr Jesus Barreto de Melo Rêgo*
Affiliation:
Immunomodulation and New Therapy Approaches Laboratory (LINAT), Biochemistry Department, Federal University of Pernambuco (UFPE), Cidade Universitária, Recife, Pernambuco 50670-901, Brazil Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Therapeutic Innovation Research Center– Suelly Galdino (NUPIT-SG), Biochemistry Department, Federal University of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, Pernambuco 50670-901, Brazil
*
*Author for correspondence: Moacyr Jesus Barreto de Melo Rêgo, E-mail: [email protected]
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Abstract

Hypoxia and nutrient deprivation are responsible for inducing malignant behavior in neoplastic cells. In these conditions, metabolic stress leads the cells to enhance their autophagic flux and to activate key molecules for homeostasis maintenance. Galectin-3 (Gal-3) is upregulated in pancreatic cancer and it is activated under the hypoxic atmosphere. We aimed to analyze the most effective autophagic-inducing conditions in pancreatic ductal adenocarcinoma cells and the effect exerted under these conditions in association with hypoxia on the Gal-3 expression. Gal-3 and the microtubule-associated protein light chain 3 beta (LC3) were accessed through western blot and immunofluorescence. Degradative vacuole quantification was analyzed by transmission electronic microscopy, and inhibition of Gal-3 was performed using siRNA. According to the analyses, the most effective conditions in the inducement of autophagy for PANC-1 and MIA PaCa-2 cells were nutritional deprivation and complete amino acid/glucose deprivation, respectively. PANC-1 cells presented higher Gal-3 when they were submitted to 24 h of nutritional deprivation alone and simultaneously nutritional and oxygen deprivation. Inhibition of Gal-3 causes a decrease of LC3 levels in all experimental conditions. These results confirm that Gal-3 is modulated by microenvironment factors and the possibility of Gal-3 participating in an adaptive response from PDAC cells to extreme conditions.

Keywords

hypoxiaMIA PaCa-2microenvironmentPANC-1pancreatic ductal adenocarcinoma
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 6 , December 2020 , pp. 1187 - 1197
Copyright
Copyright © Microscopy Society of America 2020

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