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Evaluation of Nanomechanical Properties of Tomato Root by Atomic Force Microscopy

Published online by Cambridge University Press:  18 June 2019

D. E. Nicolás-Álvarez ,
J. A. Andraca-Adame ,
J. J. Chanona-Pérez Open the ORCID record for J. J. Chanona-Pérez [Opens in a new window] ,
J. V. Méndez-Méndez ,
S. Cárdenas-Pérez  and
A. Rodríguez-Pulido
D. E. Nicolás-Álvarez
Affiliation:
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq. Cda. Miguel Stampa s/n, C.P. 07738, Gustavo A. Madero, CDMX, Mexico
J. A. Andraca-Adame
Affiliation:
Unidad Profesional Interdisciplinaria de Ingeniería Campus Hidalgo, Instituto Politécnico Nacional, Carretera “Pachuca-Actopan” Kilómetro 1+500, Municipio San Agustín Tlaxiaca, Hidalgo, Ciudad del Conocimiento y la Cultura, Hidalgo, Edo, Mexico
J. J. Chanona-Pérez*
Affiliation:
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq. Cda. Miguel Stampa s/n, C.P. 07738, Gustavo A. Madero, CDMX, Mexico
J. V. Méndez-Méndez
Affiliation:
Centro de Nanociencias, Micro y Nanotecnologías, Instituto Politécnico Nacional, Wilfrido Massieu s/n. UPALM, Gustavo A. Madero, 07738 CDMX, Mexico
S. Cárdenas-Pérez
Affiliation:
Chair of Geobotany and Landscape Planning, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Toruń, Poland
A. Rodríguez-Pulido
Affiliation:
Centro de Investigación en Sustentabilidad Energética y Ambiental, Universidad Autónoma del Noreste, A. C. Prolongación Constituyentes 1002, Col. Las Rusias, C.P. 87560, H. Matamoros, Tamaulipas, Mexico
*
*Author for correspondence: J.J. Chanona-Pérez, E-mail: [email protected]
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Abstract

Here, different tissue surfaces of tomato root were characterized employing atomic force microscopy on day 7 and day 21 of growth through Young's modulus and plasticity index. These parameters provide quantitative information regarding the mechanical behavior of the tomato root under fresh conditions in different locations of the cross-section of root [cell surface of the epidermis, parenchyma (Pa), and vascular bundles (Vb)]. The results show that the mechanical parameters depend on the indented region, tissue type, and growth time. Thereby, the stiffness increases in the cell surface of epidermal tissue with increasing growth time (from 9.19 ± 0.68 to 13.90 ± 1.68 MPa) and the cell surface of Pa tissue displays the opposite behavior (from 1.74 ± 0.49 to 0.48 ± 0.55); the stiffness of cell surfaces of Vb tissue changes from 10.60 ± 0.58 to 6.37 ± 0.53 MPa, all cases showed a statistical difference (p < 0.05). Viscoelastic behavior dominates the mechanical forces in the tomato root. The current study is a contribution to a better understanding of the cell mechanics behavior of different tomato root tissues during growth.

Keywords

atomic force microscopygrowth stagesnanoindentationplasticity indextomato root tissuesYoung's modulus
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 4 , August 2019 , pp. 989 - 997
Copyright
Copyright © Microscopy Society of America 2019 

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