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Electron Microscopy of Myelin Basic Protein (MBP) Organized as Planar Arrays on a Lipid Monolayer Surface: Deimination of MBP Hinders Its Organizational Properties

Published online by Cambridge University Press:  02 July 2020

N. Ishiyama ,
P. Matharu ,
I.R. Bates ,
D.D. Wood ,
M.A. Moscarello ,
N.J. Viner  and
G. Harauz
N. Ishiyama
Affiliation:
Molecular Biology and Genetics, University of Guelph, Guelph, Canada, NIG 2W1.
P. Matharu
Affiliation:
Pathology and Microbiology, University of Bristol, Bristol, UK, BS8 1TD.
I.R. Bates
Affiliation:
Molecular Biology and Genetics, University of Guelph, Guelph, Canada, NIG 2W1.
D.D. Wood
Affiliation:
Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Canada, M5G 1X6.
M.A. Moscarello
Affiliation:
Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Canada, M5G 1X6.
N.J. Viner
Affiliation:
Pathology and Microbiology, University of Bristol, Bristol, UK, BS8 1TD.
G. Harauz
Affiliation:
Molecular Biology and Genetics, University of Guelph, Guelph, Canada, NIG 2W1.
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Extract

Myelin basic protein (MBP) is one of the principal constituents of the mammalian myelin sheath. It is a basic peripheral membrane protein in the major dense line and is believed to play a structural role in maintaining myelin stability through its close association with lipids and other proteins. Immune responses to MBP have been implicated in the pathogenesis of multiple sclerosis (MS), the most common autoimmune disease of the central nervous system in North America and Northern Europe. The correlation between the severity of MS and the deimination of arginyl to citrullinyl residues in MBP was well illustrated in the acute case of MS (Marburg type) that contained MBP with 18 out of 19 arginines citrullinated.

We have studied a recombinant hexahistidine-tagged murine MBP (rmMBP, 18.5 kDa isoform) by electron microscopy of the protein organized as planar arrays on lipid monolayers that consisted of the nickel chelating lipid, l,2-dioleoyl-.yn-glycero-3-[(N(5-amino-l-carboxypentyl)iminodiacetic acid)succinyl] (Nickel salt) (Ni2+-NTADOGS), and the filler lipid, liver phosphatidylinositol (PI).

Type
Electron Cryomicroscopy of Macromolecules
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 250 - 251
Copyright
Copyright © Microscopy Society of America

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References

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