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Electron Microscopy Findings in N-Methyl-N-Nitrosourea-Induced Mammary Tumors

Published online by Cambridge University Press:  22 September 2016

Ana I. Faustino-Rocha ,
Ana M. Calado ,
Adelina Gama ,
Rita Ferreira ,
Mário Ginja  and
Paula A. Oliveira
Ana I. Faustino-Rocha*
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal Animal and Veterinary Research Center (CECAV), UTAD, 5001-801, Vila Real, Portugal Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Department of Chemistry, Mass Spectrometry Center, University of Aveiro, 3810-193, Aveiro, Portugal
Ana M. Calado
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal
Adelina Gama
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Animal and Veterinary Research Center (CECAV), UTAD, 5001-801, Vila Real, Portugal
Rita Ferreira
Affiliation:
Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Department of Chemistry, Mass Spectrometry Center, University of Aveiro, 3810-193, Aveiro, Portugal
Mário Ginja
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal
Paula A. Oliveira
Affiliation:
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801, Vila Real, Portugal Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5001-801, Vila Real, Portugal
*
*Corresponding author. [email protected]
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Abstract

Although the rat model of mammary tumors chemically induced by N-methyl-N-nitrosourea (MNU) has been frequently used by several research teams, there is a lack of ultrastructural studies in this field. The main aim of this work was to perform an ultrastructural characterization of MNU-induced mammary tumors in female rats. Some alterations previously reported in human mammary tumors, such as nucleus size and shape, accumulation of heterochromatin in the perinuclear region, and interdigitating cytoplasmic processes between cancer cells were also observed in MNU-induced mammary tumors. Although a low number of samples were analyzed by transmission electron microscopy in the present study, we consider that it may contribute to a better understanding of MNU-induced mammary carcinogenesis in a rat model. The ultrastructural characteristics of the two most frequently diagnosed mammary carcinomas described in the present work can be useful to differentiate them from other histological patterns. In addition, the loss of cytoplasm in neoplastic cells and formation of vacuoles were described.

Keywords

N-methyl-N-nitrosourea-induced mammary tumorratSprague-Dawleytransmission electron microscopyultrastructure
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 5 , October 2016 , pp. 1056 - 1061
Copyright
© Microscopy Society of America 2016 

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