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Effects of Mesua ferrea Leaf and Fruit Extracts on Growth and Morphology of Staphylococcus aureus

Published online by Cambridge University Press:  18 January 2013

Claira Arul Aruldass ,
Mani Maran Marimuthu ,
Surash Ramanathan ,
Sharif Mahsufi Mansor  and
Vikneswaran Murugaiyah
Claira Arul Aruldass
Affiliation:
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
Mani Maran Marimuthu
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Surash Ramanathan
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Sharif Mahsufi Mansor
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Vikneswaran Murugaiyah*
Affiliation:
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
*
*Corresponding author. E-mail: [email protected]
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Abstract

Mesua ferrea is traditionally used for treating bleeding piles, fever, and renal diseases. It has been reported to have antimircobial activity. In the present study, antibacterial efficacy of leaf and fruit extracts on the growth and morphology of Staphylococcus aureus is evaluated. Both extracts display good antibacterial activity against S. aureus with a minimum inhibition concentration of 0.048 mg/mL. Both extracts are bacteriostatic at a minimum bacteriostatic concentration of 0.39 mg/mL. The bacteriostatic activity lasts for 24 h, and then cells start to grow as normal as shown in time-kill analysis. Scanning electron microscopy study indicated potential detrimental effect of the extracts of leaf and fruits of M. ferrea on the morphology of S. aureus. The treatment with the extracts caused extensive lysis of the cells, leakage of intracellular constituents, and aggregation of cytoplasmic contents forming an open meshwork of the matrix.

Keywords

Staphylococcus aureusMesua ferreatime-kill analysisbacteriostaticscanning electron microscopymorphology
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 254 - 260
Copyright
Copyright © Microscopy Society of America 2013

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