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Development of a Philips cryo-TEM provided with a liquid helium cooled tilt stage and a vacuum transfer system
Published online by Cambridge University Press: 02 July 2020
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In order to reduce beam damage, biological TEM specimens are often observed at temperatures close to the boiling point of liquid nitrogen (77 K). Recently, encouraging results on single particles as well as on 2D crystals have appeared, derived from images taken near liquid helium temperature (4 K), in dedicated TEMs. At these temperatures the high resolution frequencies are much better preserved, increasing the allowable dose and thus the signal to noise ratio.4 Here we present the design of a new dedicated Philips He-TEM which combines the full functionality of a CM300 TWIN with a vacuum transfer system and a liquid helium cooled specimen holder.
A schematic overview of the Cryo-TEM is shown in figure 1. The key differences compared to a standard CM microscope are: 1) The tip of the specimen rod is cooled below 10 K and the rod itself cannot be taken out of the goniometer (CompuStage). 2) The specimen enters the column on the opposite side.
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- Advances in Instrumentation and Performance
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- Copyright © Microscopy Society of America