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Demonstrating Angiogenesis In a Human Testicular Malignant Tumor Using the Scid-Maus Model. Intravital Video Microscopy and Scanning Electron Microscopy of Vascular Corrosion Casts

Published online by Cambridge University Press:  02 July 2020

S. Aharinejad ,
M. Schlag ,
H. Abri ,
M. Fink ,
F. Nourani ,
S. Nedwed  and
P. Schlatter
S. Aharinejad
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
M. Schlag
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
H. Abri
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
M. Fink
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
F. Nourani
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
S. Nedwed
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
P. Schlatter
Affiliation:
Microcirculation Laboratory, Dept. of Anatomy, University of Vienna, A-, 1090ViennaAustria
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Extract

Growth of malignant tumors relies on development of new vessels from the existing vessels, a process called angiogenesis. Little is known about the pattern of chronic vascular growth in the human germ cell testicular cancer. To shed light on this topic, we injected human CRL-2073 testicular cancer cells or saline (sham treated mice) into the testes of SCID-mice and studied the growth of the tumor's vasculature using intravital video microscopy (IVM) on days 7, 14, 21, 28 and 37 after injection. On each day, 4 mice were studied. To better identify microvessels, fluorescein isothiocyanate-dextran (FITC) was injected i.v. prior to IVM. After IVM, one testis was cast with Mercox and studied in a scanning electron microscope (SEM), the other was used for histology. IVM images were transferred to the computer and analyzed using the Lucia morphometry software.

Type
Applications and Advances In Vascular Corrosion Casting In Microvascular Research
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1196 - 1197
Copyright
Copyright © Microscopy Society of America

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