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A Cross-Correlation Method for Merging Electron Crystallographic Image Data

Published online by Cambridge University Press:  08 August 2003

Irene M. Thomas  and
Michael F. Schmid
Irene M. Thomas
Affiliation:
Verna and Marrs McLean Department of Biochemistry and W. M. Keck Center for Computational Biology, Baylor College of Medicine, Houston, TX 77030
Michael F. Schmid
Affiliation:
Verna and Marrs McLean Department of Biochemistry and W. M. Keck Center for Computational Biology, Baylor College of Medicine, Houston, TX 77030
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Abstract

An efficient method of merging images from electron crystallographic data has been developed that is several orders of magnitude faster than the method of phase shift origin search. The results of this new cross-correlation method are compared with the phase residual in terms of speed, consistency with the phase residual method, and the ease of incorporating symmetry constraints along with the image-to-image merging. The program is further accelerated by a more efficient sorting procedure.

Keywords

electron crystallographyfast Fourier transformimage processingsymmetryphase residualthree-dimensional reconstructionX-ray crystallography
Type
Research Article
Information
Microscopy and Microanalysis , Volume 1 , Issue 4 , August 1995 , pp. 167 - 173
Copyright
© 1995 Microscopy Society of America

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