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Contribution of Light and Electron Microscopy to Identification of Bark from Frangula azorica, an Azorean Medicinal Plant

Published online by Cambridge University Press:  09 September 2015

Maryam Malmir ,
Cátia Curica ,
Elsa T. Gomes ,
Rita Serrano  and
Olga Silva
Maryam Malmir
Affiliation:
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisbon, Portugal
Cátia Curica
Affiliation:
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisbon, Portugal
Elsa T. Gomes
Affiliation:
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisbon, Portugal
Rita Serrano
Affiliation:
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisbon, Portugal
Olga Silva*
Affiliation:
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisbon, Portugal
*
*Corresponding author. [email protected]
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Abstract

Frangula azorica V. Grubow is a Macaronesian flora medicinal plant, endemic from Azores islands and inscribed on the International Union for the Conservation of Nature (IUCN) Red List of Threatened Species. This species, known as “sanguinho,” belongs to the family Rhamnaceae, the same as Frangula alnus Mill. and Frangula purshiana (DC.) J. G. Cooper, two widely used official laxative herbal medicines of the western Pharmacopoeias constituted by the dried barks of each species. Morphological and chemical studies on F. azorica dried bark are scarce although it is potentially recognized as a Portuguese laxative herbal medicine. Macroscopically, the bark occurs in quills or nearly flat pieces. A channeled external surface with transversely elongated lenticels is characteristic. When the outer phellem layer is removed, a bright purple inner phellem layer is disclosed. Light and electron microscopy observations revealed flattened phellem cells with slightly thickened walls, cortical parenchyma with secretory ducts and groups of sclereids, phloem with groups of fibers and sheaths of parenchymatous cells containing druses or more frequently prismatic crystals of calcium oxalate, and parenchymatous medullary rays one to three cells wide with spherical starch grains. Observation of these botanical characteristics must be included in quality monographs of F. azorica bark herbal medicine.

Keywords

botanical identificationherbal medicinesFrangula azoricaRhamnaceaelight microscopyscanning electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 5 , October 2015 , pp. 1296 - 1303
Copyright
© Microscopy Society of America 2015 

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References

Applequist, W. (2006). The Identification of Medicinal Plants. A Handbook of the Morphology of Botanicals in Commerce. Missouri: Missouri Botanical Garden Press.Google Scholar
Coutinho, A.P. (1939). Flora de Portugal (Plantas Vasculares). Lisboa: Bertrand (Irmãos) Ltd.Google Scholar
Cresseri, A., Peruto, I. & Longo, R. (1966). Biologische Wertbestimmung der Laxativen Wirkung der Polyhydroxy-Anthrachinone aus der Rinde Von Rhamnus Frangula L. Arch Pharm (Weinheim) 299, 615–618.CrossRefGoogle Scholar
Curica, C., Serrano, R., Gomes, E.T. & Silva, O. (2008). Identification of Frangula azorica bark a Portuguese medicinal drug. Planta Med 74, 242.CrossRefGoogle Scholar
Curica, C., Serrano, R., Gomes, E.T. & Silva, O. (2009). Morphological characterization of Frangula azorica bark as a potential herbal drug. Microsc Microanal 15(Suppl 3), 11–12.CrossRefGoogle Scholar
EDQM (2010). European Pharmacopoeia 7th ed. vol.1: General Monographs. Strasbourg, France: European Directorate for the Quality of Medicines, Council of Europe.Google Scholar
Fairbairn, J.W. & Mital, V.K. (1958). Vegetable purgatives containing anthracene derivatives. IX. An Aloin-like substances in Rhamnus purshiana DC. J Pharm Pharmacol 10, T217–T222.CrossRefGoogle ScholarPubMed
Fairbairn, J.W. & Simic, S. (1960). Vegetable purgatives containing anthracene derivatives. XI. Further work on the aloin-like substance of Rhamnus purshiana DC. J Pharm Pharmacol 12, T45–T51.CrossRefGoogle Scholar
Franco, J.A. (1971). Nova Flora de Portugal. vol. I. Lisboa: author’s edition.Google Scholar
INFARMED (2008). Farmacopeia Portuguesa, 9ª ed. Lisboa: Infarmed, Autoridade Nacional do Medicamento e Produtos de Saúde, I. P.Google Scholar
Jackson, B.P. & Snowdon, D.W. (1990). Atlas of Microscopy (pp. 102). London: Belhaven Press, A Division of Printer Publishers.Google Scholar
Press, J.R. & Short, M.J. (Eds.) 1994). Flora of Madeira. London: Natural History Museum/HMSO.Google Scholar
Serrano, R., Da Silva, G. & Silva, O. (2010). Application of light and scanning electron microscopy in the identification of herbal medicines. In Microscopy: Science, Technology, Applications and Education, (Microscopy Book Series, Number 4), vol. 1. Méndez-Vilas A. & Díaz J. (Eds.), pp. 182–190. Badajoz: Formatex Research Center.Google Scholar
Silva, O. (1993). Internal Report, VIII Scientific Expedition Faial 1993 of the Department of Biology of University of Azores. Ecology, Taxonomy and Conservation. Faculty of Pharmacy, Universidade de Lisboa.Google Scholar
Silva, O. (1994). Internal Report, IX Scientific Expedition Terceira 1994 of the Department of Biology of University of Azores. Ecology, Taxonomy and Conservation. Faculty of Pharmacy, Universidade de Lisboa.Google Scholar
Stahl, E. (1973). Drug Analysis by Chromatography and Microscopy (p. 72). Michigan, USA: Ann Arbor Science Publishers Inc.Google Scholar
Upton, R., Graff, A., Jolliffe, G., Laenger, R. & Williamson, E. (Eds.) (2011). American Herbal Pharmacopoeia: Botanical Pharmacognosy – Microscopic Characterization of Botanical Medicines. Boca Raton: American Herbal Pharmacopoeia/CRC Press.Google Scholar
WCMC (1998). Frangula azorica. The IUCN Red List of Threatened Species. Version 2014.3. Available at www.iucnredlist.org (retrieved April 21, 2015).Google Scholar
WHO (2004 a). Cortex Rhamni purshianae. In WHO Monographs on Selected Medicinal Plants vol. 2, pp. 259–268. Geneva, Switzerland: World Health Organization.Google Scholar
WHO (2004 b). Cortex frangulae. In WHO Monographs on Selected Medicinal Plants vol. 2, pp. 114–123. Geneva, Switzerland: World Health Organization.Google Scholar