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CIV, An Iridovirus With a Pseudo T=147 Capsid Structure

Published online by Cambridge University Press:  02 July 2020

X. Yan ,
N. H. Olson ,
M. Bergoin ,
M. G. Rossmann  and
T. S. Baker
X. Yan
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
N. H. Olson
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
M. Bergoin
Affiliation:
Lab of Comparative Pathology, University of Montpellier II, Montpellier, France34095
M. G. Rossmann
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
T. S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN47907-1392
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Extract

Chilo iridescent virus (CIV), the type species of the genus Iridovirusin the family Iridoviridae,was first isolated from the rice stem borer, Chilo suppressalis,a member of Lepidoptera insect order. ‘2 CIV is being considered as a potential pest control agent because of its wide host range which includes leafhoppers and some arthropods. The virion (∼lxl09 daltons) encapsidates a 209 kbp, dsDNA genome within its fiber-covered, icosahedral shell. The virion is composed of about 26 structural proteins, two (P50 and P'50, 50kDa each) of which are the major capsid proteins. P50 migrates as a monomer in either a reducing or non-reducing SDS-PAGE gel and is exposed to the surface, whereas P'50 exists as a disulfide-bond, stabilized trimer in a non-reducing gel and is only partially exposed. Virions contain 9% lipid present as a lipid bilayer within the protein capsid.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1134 - 1135
Copyright
Copyright © Microscopy Society of America

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References

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10. We thank R. Ashmore for assistance with programming. Work supported in part by grants from the NIH (GM-33050), NSF (MCR-9527131), and a shared equipment grant from NSF (BIR 9112921) toT.S.B.Google Scholar