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The Centrosome: Three-Dimensional Structure of a Cell Organelle at 40A Resolution Obtained by Electrontomography

Published online by Cambridge University Press:  02 July 2020

T. Ruiz ,
M. Radermacher ,
B. Rath ,
C. Rieder  and
M. Bornens
T. Ruiz
Affiliation:
Institut Curie, Section de Recherche, 26 rue d'Ulm, 75231ParisCedex 05, France
M. Radermacher
Affiliation:
Max-Planck-Institut fur Biophysik, Heinrich-Hoffmann-Str. 7, Frankfurt/M., FRG
B. Rath
Affiliation:
Wadsworth Center, New York State Dept. of Health, Albany, N.Y. 12201-0509, USA
C. Rieder
Affiliation:
Wadsworth Center, New York State Dept. of Health, Albany, N.Y. 12201-0509, USA
M. Bornens
Affiliation:
Institut Curie, Section de Recherche, 26 rue d'Ulm, 75231ParisCedex 05, France
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Extract

The organization of the microtubule network of eukariotic cells is controlled by the centrosome. This organelle which duplicates once during the cell cycle comprises two centrioles and a dense filamentous material called pericentriolar material. The centrioles are cylinders of ≈ 0.25 μm in diameter and ≈ 0.5μm in length and are composed of 9 microtubule triplets at the proximal end and 9 doublets at the distal end. For the last 50 years the centrosome and the centrioles in particular have been the object of many optical and electron microscopy studies. But, in spite of their highly symmetric organization, their fine structure is still not well understood. The reconstruction presented here is a substantial step forward towards a more detailed understanding of their structure.

Centrosomes were isolated from a human lymphoblastoma cell line (1). 10 nm gold particles were applied to formvar-carbon coated grids to provide markers for the alignment procedure (2,3). Centrosomes were centrifuged onto the grids and negatively stained.

Type
Unique Approaches in Imaging, Computation and Communication for Characterization of the 3D Cell & Organelles I
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 436 - 437
Copyright
Copyright © Microscopy Society of America

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References

References:

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(5) The work was partially supported by grant NIH - P41 RR01219 (P.I. C.Rieder)Google Scholar