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An Insight into the Potential Parasitological Effect of Schistosoma mansoni Antigens in Infected Mice: Prophylactic Role of Cercarial Antigen

Published online by Cambridge University Press:  22 June 2020

Sara S. Abdel-Hakeem Open the ORCID record for Sara S. Abdel-Hakeem [Opens in a new window] ,
Mahmoud A. Abdel-Samiee  and
Gamal H. Abed
Sara S. Abdel-Hakeem*
Affiliation:
Parasitology Lab., Zoology Department, Faculty of Science, Assiut University, Assiut71516, Egypt
Mahmoud A. Abdel-Samiee
Affiliation:
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Gamal H. Abed
Affiliation:
Parasitology Lab., Zoology Department, Faculty of Science, Assiut University, Assiut71516, Egypt
*
*Author for correspondence: Sara S. Abdel-Hakeem, E-mail: [email protected]
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Abstract

Schistosomiasis is a multifactorial disease that includes environmental, behavioral, parasitic, vector, and host factors. This study aimed to assess the protective effect of single and polyvalent antigens from cercarial antigen preparations (CAPs), soluble worm antigen preparations (SWAPs), and soluble egg antigens (SEAs) which were used as candidate vaccines in an experimental model of Schistosoma mansoni-infected mice. The efficiency of the antigens was tested by determining their effects on fecal egg count, egg viability analysis, and tissue egg counts. Histological and morphometric analyses of granulomas in liver and intestine tissues were performed. In the present study, all immunized groups showed a significant reduction in the average fecal egg count and tissue egg load compared with infected mice. The most substantial reduction in fecal egg count was observed in the combined vaccinated group (23.23 ± 3.2). The group vaccinated with CAP before infection showed the highest reduction in tissue egg load (liver and intestine: 85.22 and 91.70%, respectively). Immunized animals showed a highly significant reduction in the numbers of hepatic granulomas compared with the infected non-immunized group. In conclusion, combining these different antigens (CAP, SWAP, and SEA) augments the protective immunity compared with other immunized groups.

Keywords

antigenscombinedegg countSchistosoma
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 4 , August 2020 , pp. 708 - 716
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Copyright
Copyright © Microscopy Society of America 2020

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References

Abdel-Hakeem, S, Abdel-Samiee, M & Abed, G (2019 a). Prediction of long-term humoral and cellular immune response: A comparison of three Schistosoma mansoni Antigens. Res Rev: J Med Chem 1(1), 10–25.Google Scholar
Abdel-Hakeem, SS, Mahmoud, GA-E & Abdel-Hafeez, HH (2019 b). Evaluation and microanalysis of parasitic and bacterial agents of Egyptian fresh Sushi, Salmo salar. Microsc Microanal 25(6), 1498–1508.CrossRefGoogle ScholarPubMed
Alves, CC, Araujo, N, Santos, VCFd, Couto, FB, Assis, NRG, Morais, SB & Fonseca, CT (2015). Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection. PLOS Neglected Trop Dis 9(2), 1–20.CrossRefGoogle Scholar
Amsalu, G, Mekonnen, Z & Erko, B (2015). A new focus of Schistosomiasis mansoni in Hayk Town, northeastern Ethiopia. BMC Res Notes 8, 1.CrossRefGoogle ScholarPubMed
Bancroft, J & Stevens, A (1982). Theory and Practice of Histological Technique. London: Livingston.Google Scholar
Boros, DL & Warren, KS (1970). Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med 132(3), 488–507.CrossRefGoogle ScholarPubMed
Capron, A, Riveau, G, Grzych, JM, Boulanger, D, Capron, M & Pierce, R (1995). Development of a vaccine strategy against human and bovine schistosomiasis: Background and update. Mem Inst Oswaldo Cruz 90(2), 235–240.CrossRefGoogle ScholarPubMed
Carter, CE & Colley, DE (1979). Partial purification and characterization of Schistosoma mansoni soluble egg antigen with Con A-Sepharose chromatography. J Immunol 122, 2204–2209.Google ScholarPubMed
Cheesbrough, M (1987). Medical Laboratory Manual for Tropical Countries, 2nd edn., vol. 1. 14 Bevills Close, Doddington: Cambridgeshire..Google Scholar
Cheever, A (1968). Conditions affecting the accuracy of potassium hydroxide digestion techniques for couting Schistosoma mansoni eggs in tissues. Bull World Health Organ 39, 328–331.Google Scholar
Colley, DG, Hieny, SE, Bartholomew, RK & Cook, JA (1977). Immune responses during human Schistosomiasis mansoni. III. Regulatory effect of patient sera on human lymphocyte blastogenic responses to schistosome antigen preparations. Am J Trop Med Hyg 26(5), 917–925.CrossRefGoogle ScholarPubMed
Dajem, SMB, Ibrahim, EH, Al-Quraishy, SA & Mostafa, OM (2011). Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR. Gene 485(2), 69–72.CrossRefGoogle Scholar
Elsenoor, KJ, Kuijk, LM, Lindhorst, TK, Cummings, RD & van Die, I (2015). Schistosoma mansoni soluble egg antigens induce expression of the negative regulators SOCS1 and SHP1 in human dendritic cells via interaction with the mannose receptor. PLOS One 10(4). e0124089.Google Scholar
Etewa, SE, El-Aal, NFA, Abdel-Rahman, SA & El-Shafey, MA (2014). Parasitological evaluation of potential candidate vaccines in Schistosoma mansoni-infected mice. J Parasitol Vector Biol 6(2), 23–30.Google Scholar
Eugene, HG, Möllegåard, I, Duncan, JF Jr., Sumner, MP, Stek, M Jr. & Hunter, KW Jr. (1987). Early diagnosis of Schistosoma mansoni in mice using assays directed against cercarial antigens isolated by hydrophobic chromatography. J Parasitol, 73 (2), 351–362.Google Scholar
Ewaisha, RE, Bahey-El-Din, M, Mossallam, SF, Amer, EI, Aboushleib, HM & Khalil, AM (2014). Combination of the two schistosomal antigens Sm14 and Sm29 elicits significant protection against experimental Schistosoma mansoni infection. Exp Parasitol 145, 51–60.CrossRefGoogle ScholarPubMed
Ismail, OA (2005). Study of the efficacy of adult worm, cercarial and egg antigens in protection against experimental intestinal schistosomiasis. Dissertation, Suez Canal University, Faculty of Medicine. Egypt.Google Scholar
Ivory, C & Chadee, K (2004). DNA vaccines: Designing strategies against parasitic infections. Genet Vaccines Ther 2(1), 17.CrossRefGoogle ScholarPubMed
Kamel, F, Wright, WW, Mock, EJ & Frankel, AI (1977). The influence of mating and related stimuli on plasma levels of luteinizing hormone, follicle stimulating hormone, prolactin, and testosterone in the male rat. Endocrinology 101(2), 421–429.CrossRefGoogle ScholarPubMed
Kaplan, MH, Whitfield, JR, Boros, DL & Grusby, MJ (1998). Th2 cells are required for the Schistosoma mansoni egg-induced granulomatous response. J Immunol 160(4), 1850–1856.Google ScholarPubMed
Katz, SP & Colley, DG (1976). Induction of cellular and humoral immunological responsiveness to a soluble cercarial antigen preparation from Schistosoma mansoni. Infect Immun 14(2), 502–508.CrossRefGoogle ScholarPubMed
Khalifa, RMA, Elnadi, NA, Omran, EK & Abdel-Tawab, RA (2011). Immunological response and the probability of production of vaccine for schistosome parasites. Egypt J Med Sci 32(2), 547–570.Google Scholar
Lochmatter, C, Schneider, CL, Ingram, K, Keiser, J & Schifferli, JA (2012). Schistosoma mansoni tetraspanning orphan receptor (SmTOR): A new vaccine candidate against schistosomiasis. ClinExp Immunol 170(3), 342–357.Google ScholarPubMed
McManus, DP & Loukas, A (2008). Current status of vaccines for schistosomiasis. Clin Microbiol Rev 21(1), 225–242.CrossRefGoogle ScholarPubMed
Melvin, DM & Brooke, MM (1982). Laboratory procedures for the diagnosis of intestinal parasites, 3rd ed US Department of Health, Education, and Welfare publication (CDC) 82-8282. US Printing Office, Washington, DC.Google Scholar
Mostafa, OM, Eid, RA & Adly, MA (2011). Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice. Parasitol Res 109(2), 395–403.CrossRefGoogle ScholarPubMed
Mutapi, F (2015). Changing policy and practice in the control of pediatric schistosomiasis. Pediatrics 135(3), 536–544.CrossRefGoogle ScholarPubMed
Nascimento, E, Leao, IC, Pereira, VR, Gomes, YM, Chikhlikar, P, August, T & Lucena-Silva, N (2002). Protective immunity of single and multi-antigen DNA vaccines against schistosomiasis. Mem Inst Oswaldo Cruz 97, 105–109.CrossRefGoogle ScholarPubMed
Negrão-Corrêa, D, Fittipaldi, JF, Lambertucci, JR, Teixeira, MM, de Figueiredo Antunes, CM & Carneiro, M (2014). Association of Schistosoma mansoni-specific IgG and IgE antibody production and clinical schistosomiasis status in a rural area of Minas Gerais, Brazil. PloS One 9(2), e88042.CrossRefGoogle Scholar
Pearce, EJ, Basch, PF & Sher, A (1986). Evidence that the reduced surface antigenicity of developing Schistosoma mansoni schistosomula is due to antigen shedding rather than host molecule acquisition. Parasite Immunol 8(1), 79–94.CrossRefGoogle ScholarPubMed
Pellegrino, J & Katz, N (1969). Experimental chemotherapy of schistosomiasis. IV. Oogram studies with nicarbazin, an egg-suppressive agent. Rev Inst Med Trop Sao Paulo 11(3), 215–221.Google ScholarPubMed
Reda, ES, Ouhtit, A, Abdeen, SH & El-Shabasy, EA (2012). Structural changes of Schistosoma mansoni adult worms recovered from C57BL/6 mice treated with radiation-attenuated vaccine and/or praziquantel against infection. Parasitol Res 110(2), 979–992.CrossRefGoogle ScholarPubMed
Romeih, MH, Hassan, HM, Shousha, TSA & Saber, MA (2008). Immunization against Egyptian Schistosoma mansoni infection by multivalent DNA vaccine. Acta Biochim Biophys Sin 40(4), 327–338.CrossRefGoogle ScholarPubMed
Salih, S, Bartlett, A & Voller, A (1978). Detection of antibodies by enzyme immune-assay in human Schistosoma mansoni infections: A clinical and chemotherapeutic study. Tropen Med Parasitol 29(4), 409–412.Google Scholar
Soliman, MF, El Shenawy, NS & El Arabi, SE (2008). Schistosoma mansoni: Melatonin enhances efficacy of cercarial and soluble worm antigens in the induction of protective immunity against infection in the hamster. Exp Parasitol 119(2), 291–295.CrossRefGoogle ScholarPubMed
SPSS. (1998). SPSS for Windows. Inc., Headquarters, Chicago.Google Scholar
Steinmann, P, Keiser, J, Bos, R, Tanner, M & Utzinger, J (2006). Schistosomiasis and water resources development: Systematic review, meta-analysis, and estimates of people at risk. Lancet Infect Dis 6(7), 411–425.CrossRefGoogle ScholarPubMed
Stireswalt, MA (1974). Schistosoma mansoni: Cercaria to schistosomule. Adv Parasitol 12, 115–182.CrossRefGoogle Scholar
Tendler, M, Pinto, RM, Lima, AO, Gebara, G & Katz, N (1986). Schistosoma mansoni: Vaccination with adult worm antigens. Int J Parasitol 16(4), 347–352.CrossRefGoogle ScholarPubMed