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3d Structure Of Periodic Cubic-Phase Inner Membranes In Mitochondria of Chaos Carolinensis

Published online by Cambridge University Press:  02 July 2020

Y. Deng ,
M. Mieczkowski ,
M. Marko ,
K. Buttle ,
B.K. Rath  and
C.A. Mannella
Y. Deng
Affiliation:
Department of Biophysical Sciences, University at Buffalo (SUNY)
M. Mieczkowski
Affiliation:
Department of Biophysical Sciences, University at Buffalo (SUNY)
M. Marko
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509
K. Buttle
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509
B.K. Rath
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509
C.A. Mannella
Affiliation:
Biological Microscopy and Image Reconstruction Resource, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509 Department of Biomedical Sciences, School of Public Health,University at Albany (SUNY)
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Extract

The mitochondrial inner membrane contains the machinery of oxidative phosphorylation. This membrane has invaginations called cristae which vary widely in shape between organisms and between tissues in the same organism. Electron microscopic tomography indicates that, despite this pleiomorphism, there is a common design feature, namely, the cristal membranes connect to each other and to the periphery of the inner membrane by tubular regions 30-40 nm in diameter. This finding has important implications for the internal diffusion of ions, metabolites and macromolecules within mitochondria.

In some types of mitochondria, the cristae exhibit periodicity.1 In the case of the amoeba Chaos carolinensis, detailed analysis and modeling of thin-section images of mitochondria in starved cells indicate that the highly curved cristae correspond to periodic cubic surfaces. We are undertaking electron microscopic tomographic and crystallographic approaches to more thoroughly characterize these membrane phases and, in particular, establish the continuity of the internal compartments which they define.

Type
Unique Approaches In Imaging, Computation and Communication for Characterization of the 3D Cell & Organelles I
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 432 - 433
Copyright
Copyright © Microscopy Society of America

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References

1.Munn, E.A., The Structure of Mitochondria (1974) Academic Press.CrossRefGoogle Scholar
2.Mannella, C.A. et al., Microsc. Res. Tech. 27(1994)278.CrossRefGoogle Scholar
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6.Deng, Y., PhD thesis (1998), University at Buffalo (SUNY)Google Scholar
7. This research is funded by NSF grant MCB-9506113 and utilizes the facilities of the Biological Microscopy and Image Reconstruction Resource which is funded by NIH grant RR01219 and NSF grant BIR-9219043.Google Scholar