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2-Photon Excitation Laser Scanning Microscopy for High Resolution Imaging In Scattering Biological Tissues: Applications to Neuroscience

Published online by Cambridge University Press:  02 July 2020

K. Svoboda ,
B.J. Burbach ,
B. Lendvai ,
Z.F. Mainen ,
M. Maravall ,
E. Nimchinsky ,
P. O’Brien ,
B. Sabatini  and
E. Stern
K. Svoboda
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
B.J. Burbach
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
B. Lendvai
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
Z.F. Mainen
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
M. Maravall
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
E. Nimchinsky
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
P. O’Brien
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
B. Sabatini
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
E. Stern
Affiliation:
Center for Learning and Memory Cold Spring Harbor Laboratory Cold Spring Harbor, NY11724
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Extract

Optical microscopy has been at the center of biological research, and neuroscience in particular, for a long time. For example, over one hundred years ago advances in microscopical techniques directly led to the discovery that neurons are in fact cells (the so-called ‘neuron doctrine’), and subsequently to the description of the high-resolution structure of the nervous system. More recently, the development of fluorescent indicators of cellular function, including Ca2+, Na+, and pH indicators, has made it possible to image not only the structure of neurons but also aspects of their function. The most important of these has been the measurement of intracellular free calcium concentration, [Ca2+]. Calcium is an important messenger molecule because it couples electrical excitation to biochemical signaling cascades. In addition, in neurons [Ca2+] is also a good indicator of local electrical excitation and thus [Ca2+] imaging allows the measurement of cellular excitability in subcellular compartments that are inaccessible to other methods.

Type
Multi Photon Excitation Microscopy: The Next Generation
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1058 - 1059
Copyright
Copyright © Microscopy Society of America

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