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Aberration Correction to Optimize the Performance of Two-Photon Fluorescence Microscopy Using the Genetic Algorithm

Published online by Cambridge University Press:  25 January 2022

Wei Yan Open the ORCID record for Wei Yan [Opens in a new window] ,
Yangrui Huang ,
Luwei Wang ,
Yong Guo ,
Jin Li ,
Yinru Zhu ,
Zhigang Yang  and
Junle Qu
Wei Yan
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Yangrui Huang
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Luwei Wang*
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Yong Guo
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Jin Li
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Yinru Zhu
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Zhigang Yang
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
Junle Qu
Affiliation:
Center for Biomedical Photonics & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China
*
*Corresponding author: Luwei Wang, E-mail: [email protected]
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Abstract

Due to less light scattering and a better signal-to-noise ratio in deep imaging, two-photon fluorescence microscopy (TPFM) has been widely used in biomedical photonics since its advent. However, optical aberrations degrade the performance of TPFM in terms of the signal intensity and the imaging depth and therefore restrict its application. Here, we introduce adaptive optics based on the genetic algorithm to detect the distorted wavefront of the excitation laser beam and then perform aberration correction to optimize the performance of TPFM. By using a spatial light modulator as the wavefront controller, the correction phase is obtained through a signal feedback loop and a process of natural selection. The experimental results show that the signal intensity and imaging depth of TPFM are improved after aberration correction. Finally, the method was applied to two-photon fluorescence lifetime imaging, which helps to improve the signal-to-noise ratio and the accuracy of lifetime analysis. Furthermore, the method can also be implemented in other experiments, such as three-photon microscopy, light-sheet microscopy, and super-resolution microscopy.

Keywords

adaptive opticsgenetic algorithmoptical aberrationtwo-photon microscopy
Type
Software and Instrumentation
Information
Microscopy and Microanalysis , Volume 28 , Issue 2 , April 2022 , pp. 383 - 389
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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References

Albert, O, Sherman, L, Mourou, G, Norris, TB & Vdovin, G (2000). Smart microscope: An adaptive optics learning system for aberration correction in multiphoton confocal microscopy. Opt Lett 25(1), 52–54.CrossRefGoogle ScholarPubMed
Amézquita, R, Rincón, O & Torres, YM (2010). Aberration compensation using a spatial light modulator LCD. J Phys: Conf Ser 274(1), 012111.Google Scholar
Benech, A, Ng, R, Huang, J, Guo, Y & Jian, Y (2020). Depth-resolved optimization of real-time sensorless adaptive-optics optical coherence tomography. Opt Lett 45(9), 2612–2615.Google Scholar
Berezin, MY & Achilefu, S (2010). Fluorescence lifetime measurements and biological imaging. Chem Rev 110(5), 2641–2684.CrossRefGoogle ScholarPubMed
Booth, MJ (2014). Adaptive optical microscopy: The ongoing quest for a perfect image. Light Sci Appl 3, e165.CrossRefGoogle Scholar
Braat, J, Dirksen, P, Janssen, A, Haver, SV & Van, D (2005). Extended Nijboer–Zernike approach to aberration and birefringence retrieval in a high-numerical-aperture optical system. J Opt Soc Am A 22(12), 2635–2650.CrossRefGoogle Scholar
Bueno, JM, Martin, S, Stefano, B & Pablo, A (2018). Wavefront correction in two-photon microscopy with a multi-actuator adaptive lens. Opt Express 26(11), 14278–14287.CrossRefGoogle ScholarPubMed
Cha, JW, Ballesta, J & So, P (2010). Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy. J Biomed Opt 15(4), 046022.CrossRefGoogle ScholarPubMed
Chen, W, Rui, L, Milkie, DE, Sun, W, Tan, Z, Kerlin, A, Chen, TW, Kim, DS & Ji, N (2014). Multiplexed aberration measurement for deep tissue imaging. Nat Methods 11, 1037–1040.Google Scholar
Denk, W, Strickler, JH & Webb, WW (1990). Two-photon laser scanning fluorescence microscopy. Science 248(4951), 73–76.CrossRefGoogle ScholarPubMed
El-Agmy, R, Bulte, H, Greenaway, AH & Reid, DT (2005). Adaptive beam profile control using a simulated annealing algorithm. Opt Express 13(16), 6085–6091.CrossRefGoogle ScholarPubMed
Fang, YC, Tsai, CM, Macdonald, J & Pai, YC (2007). Eliminating chromatic aberration in Gauss-type lens design using a novel genetic algorithm. Appl Opt 46(13), 2401–2410.CrossRefGoogle ScholarPubMed
Helmchen, F & Denk, W (2005). Deep tissue two-photon microscopy. Nat Methods 2(12), 932–940.CrossRefGoogle ScholarPubMed
Hovhannisyan, VA, Su, PJ & Dong, CY (2008). Characterization of optical-aberration-induced lateral and axial image inhomogeneity in multiphoton microscopy. J Biomed Opt 13(4), 044023.CrossRefGoogle ScholarPubMed
Lin, CD, Anderson-Cook, CM, Hamada, MS, Moore, LM & Sitter, RR (2015). Using genetic algorithms to design experiments: A review. Qual Reliab Eng Int 31(2), 155–167.CrossRefGoogle Scholar
Manns, F, Söderberg, PG, Ho, A, Fernandez, EJ, Povazay, B, Hermann, B, Unterhuber, A, Sattmann, H, Prieto, PM & Leitgeb, RA (2006). Adaptive optics using a liquid crystal spatial light modulator for ultrahigh-resolution optical coherence tomography. Int Soc Opt Photonics 6138, 61380Y.Google Scholar
Marcu, L (2012). Fluorescence lifetime techniques in medical applications. Ann Biomed Eng 40(2), 304–331.CrossRefGoogle ScholarPubMed
Neil, M, Booth, MJ & Wilson, T (2000). Closed-loop aberration correction by use of a modal Zernike wave-front sensor. Opt Lett 25(15), 1083–1085.CrossRefGoogle ScholarPubMed
Noll, RJ (1976). Zernike polynomials and atmospheric turbulence. J Opt Soc Am A 66(3), 207–211.CrossRefGoogle Scholar
Perillo, EP, Mccracken, JE, Fernée, DC, Goldak, JR, Medina, FA, Miller, DR, Yeh, HC & Dunn, AK (2016). Deep in vivo two-photon microscopy with a low cost custom built mode-locked 1060 nm fiber laser. Biomed Opt Express 7(2), 324–334.CrossRefGoogle ScholarPubMed
Schmidt, JD, Goda, ME & Duncan, BD (2007). Aberration production using a high-resolution liquid-crystal spatial light modulator. Appl Opt 46(13), 2423–2433.CrossRefGoogle ScholarPubMed
Sherman, L, Ye, JY, Albert, O & Norris, TB (2002). Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror. J Microsc 206(1), 65–71.CrossRefGoogle ScholarPubMed
Tao, X, Norton, A, Kissel, M, Azucena, O & Kubby, J (2013). Adaptive optical two-photon microscopy using autofluorescent guide stars. Opt Lett 38(23), 5075–5078.CrossRefGoogle ScholarPubMed
Tischbirek, C, Birkner, A, Jia, H, Sakmann, B & Konnerth, A (2015). Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator. Proc Natl Acad Sci U S A 112(36), 11377–11382.CrossRefGoogle ScholarPubMed
Wang, K, Sun, W, Richie, CT, Harvey, BK, Betzig, E & Ji, N (2015). Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue. Nat Commun 6, 7276–7281.CrossRefGoogle ScholarPubMed
Wang, L, Yan, W, Li, R, Weng, X, Zhang, J, Yang, Z, Liu, L, Ye, T & Qu, J (2018). Aberration correction for improving the image quality in STED microscopy using the genetic algorithm. Nanophotonics 7(12), 1971–1980.CrossRefGoogle ScholarPubMed
Wei, W, Qin, S, Li, H & Zhang, B (2007). Influence of optical system aberration on precision of detecting target position in laser semi-active seeking guidance. Opt Technique 33, 142–146.Google Scholar
Yan, W, Yang, Y, Yu, T, Xu, C, Yang, L, Qu, J & Ye, T (2017). Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples. Photonics Res 5(3), 176–181.CrossRefGoogle ScholarPubMed
Yang, H, Liu, G, Rao, C, Zhang, Y & Jiang, W (2007). Combinational-deformable-mirror adaptive optics system for compensation of high-order modes of wavefront. Chin Opt Lett 5(8), 435–437.Google Scholar
Yang, P, Xu, B, Jiang, W & Chen, S (2008). A genetic algorithm used in a 61-element adaptive optical system. Front Optoelectron China 1, 263–267.CrossRefGoogle Scholar
Zhang, C, Sun, W, Mu, Q, Cao, Z, Zhang, X, Wang, S & Xuan, L (2019). Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy. Opt Commun 435, 46–53.CrossRefGoogle Scholar
Zipfel, WR, Williams, RM, Christie, R, Nikitin, AY, Hyman, BT & Webb, WW (2003). Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci U S A 100(12), 7075–7080.CrossRefGoogle ScholarPubMed
Zou, W, Qi, X & Burns, SA (2008). Wavefront-aberration sorting & correction for a dual-deformable-mirror adaptive-optics system. Opt Lett 33(22), 2602–2604.CrossRefGoogle ScholarPubMed
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