Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-19T08:41:50.107Z Has data issue: false hasContentIssue false
  • English
  • Français

Bone-like growth of hydroxyapatite in the biomimetic coating of Ti-6Al-4V alloy pretreated with protein at 25 °C

Published online by Cambridge University Press:  31 January 2011

Jui Chakraborty ,
Matjaz Mazaj ,
Renu Kapoor ,
S. Pavana Gouri ,
Nina Daneu ,
Mithlesh K. Sinha ,
Gopal Pande  and
Debabrata Basu
Jui Chakraborty*
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
Matjaz Mazaj
Affiliation:
National Institute of Chemistry, 1000 Ljubljana, Slovenia
S. Pavana Gouri
Affiliation:
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Nina Daneu
Affiliation:
Jozef Stefan Institute, S1-1000 Ljubljana, Slovenia
Mithlesh K. Sinha
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
Gopal Pande*
Affiliation:
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Debabrata Basu
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
*
a) e-mail: [email protected]
b) e-mail: [email protected]
Get access

Abstract

Commercial-grade dense Ti-6Al-4V alloy substrate was mechanically roughened, cleaned, and treated with a globular protein [bovine serum albumin (BSA)] for 4 h. Biomimetic calcium phosphate (Ca-P) coating was applied onto the above-treated substrate by immersion into simulated body fluid (SBF) at 25 °C for a period of 4 days, with periodic replacement by freshly prepared SBF at 48-h intervals. After 4 days, branched micron-sized fibers of hydroxyapatite (HAp), resembling the structure of bone, were obtained, connecting the clusters of HAp crystal plates in the coating (thickness ∼200 μm) on the substrate surface. Structural and compositional characterization of the coating was carried out using field emission scanning electron microscopy (FE-SEM) with energy-dispersive x-ray analysis unit (EDX) facility, x-ray diffraction (XRD), and Fourier transform infrared (FTIR) data. In vitro cytotoxicity (ISO 10993-5, 1999), cell adhesion assays, and phase contrast microscopy were performed using NIH 3T3 fibroblast cell lines to ascertain the bioactivity of the coated substrates, with and without protein treatment. Based on our study, we propose a correlation between a specific physical structure of the HAp coating and its biological properties.

Keywords

BiomaterialBiomimetic (assembly)Coating
Type
Articles
Information
Journal of Materials Research , Volume 24 , Issue 6 , June 2009 , pp. 2145 - 2153
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Holmes, R.E. and Hagler, H.K.: Porous hydroxylapatite as a bone grafts substitute in mandibular contour augmentation. A histo-metric study. J. Oral Maxillofac. Surg. 45, 421 (1987).CrossRefGoogle ScholarPubMed
2Isakson, S.: Aspects of Bone Healing and Substitute Incorporation: An Experimental Study in Rabbit Skull Bone Defects (Lund University, Malmo, Sweden, 1992).Google Scholar
3Liu, Y.L., Schoenaers, J., de Groot, K., Wijn, J.R. de, and Schepers, E.: Bone healing in porous implants. An experiment in sheep. J. Mater. Sci.–Mater. Med. 11, 667 (2000).Google Scholar
4Veerman, E.C.I., Suppers, R.J.F., Klein, C.P.A.T., Groot, K. de, and Nieuw, A.V.Amerongen: SDS-PAGE analysis of the protein layers adsorbing in vivo and in vitro to bone substituting materials. Biomaterials 8, 442 (1987).CrossRefGoogle Scholar
5Johnson, M.S.A., Paschalis, E., and Nancolias, G.H.: Kinetics of mineralisation, demineralisation and transformation of calcium phosphate at mineral and protein surfaces, in The Bone-Material Interface, edited by Davies, J.E. (University of Toronto Press, Toronto, Canada, 1991), pp. 68–75.Google Scholar
6Suzuki, O., Yagishta, H., Yamazaki, M., and Aoba, T.: Adsorption bovine albumin onto octacalcium phosphate and its hydrolyzates. Cells Mater. 5, 45 (1995).Google Scholar
7Chen, Y., Mak, A.F., Li, J., Wang, M., and Shum, A.W.: Formation of apatite on poly (alpha-hydroxy acid) in an accelerated biomimetic process. J. Biomed. Mater. Res. 73(1), 68 (2005).CrossRefGoogle Scholar
8Barrere, F., van Blitterswijk, C.A., Groot, K. de, and Layrolle, P.: Influence of ionic strength and carbonate on the Ca-P coating formation from SBF[notdef]5 solution. Biomaterials 23, 1921 (2002).CrossRefGoogle Scholar
9Tas, A.C. and Bhaduri, S.B.: Rapid coating of Ti-6Al-4V at room temperature with a calcium phosphate solution similar to 10[notdef]simulated body fluid. J. Mater. Res. 19(9), 2742 (2004).CrossRefGoogle Scholar
10Song, W.H., Jun, Y.K., Han, Y., and Hong, S.H.: Biomimetic apatite coating on micro-arc oxidized titania. Biomaterials 25, 3341 (2004).CrossRefGoogle ScholarPubMed
11Amstutz, H.C.: New advances in bone research. West. J. Med. 141, 71 (1984).Google ScholarPubMed
12Rosen, V. and Thies, R.S.: The BMP proteins in bone formation and repair. Trends Genet. 8, 97 (1992).CrossRefGoogle ScholarPubMed
13Urist, M.R., Lietze, A., Mizutani, H., Takagi, K., Triffittj, T., Amstutz, J., Dlange, R., Termine, J., and Finerman, G.A.M.: A bovine low molecular weight bone morphogenetic protein (BMP) fraction. Clin. Orthop. Relat. Res. 162, 219 (1982).CrossRefGoogle Scholar
14Vicsek, M. and Vicsek, T.: Fractal growth models. ERCIM News (Online Edition), No. 29, April 1997.Google Scholar
15Chakraborty, J., Bharati, S., Sinha, M.K., and Basu, D.: A process for the production of protein mediated calcium hydroxyapatite (HAp) coating on metal substrates particularly on stainless steel (316L). Patent applied to CSIR (Council of Scientific and Industrial Research, New Delhi, India) vide Indian Patent application Number 1759 DEL 2007, filing date 20.08.07.Google Scholar
16Gopalakrishna, P., Chaubey, S.K., Manogaran, P.S., and Pande, G.: Modulation of a5b1 integrin functions by the phospholipid and cholesterol contents of cell membranes. J. Cell. Biochem. 77, 517 (2000).3.0.CO;2-6>CrossRefGoogle ScholarPubMed
17Luo, Q. and Andrade, J.D.: Cooperative adsorption of proteins onto hydroxyapatite. J. Colloid Interface Sci. 200, 104 (1998).CrossRefGoogle Scholar
18Wen, H.B., de Wijn, J.R., Blitterswijk, C.A. van, and Groot, K. de: Incorporation of bovine serum albumin in calcium phosphate coating on titanium. J. Biomed. Res. 46(2), 245 (1999).3.0.CO;2-A>CrossRefGoogle ScholarPubMed
19Liu, Y., Laryolle, P., Brujn, J.D., Blitterswijk, C.V., and Groot, K.D.: Biomimetic co precipitation of calcium phosphate and bovine serum albumin on titanium alloy. J. Biomed. Mater. Res. 57(3), 327 (2001).3.0.CO;2-J>CrossRefGoogle Scholar