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Analysis of polyunsaturated fatty acid composition of Strongyloides ratti in relation to development

Published online by Cambridge University Press:  05 June 2009

Takeya Minematsu ,
Seietsu Yamazaki ,
Yoshinori Uji ,
Hiroaki Okabe ,
Masataka Korenaga  and
Isao Tada
Takeya Minematsu
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Seietsu Yamazaki
Affiliation:
Analytical Chemistry Group, Tsukuba Research Center, Mitsubishi Petrochemical Co., Ltd. 8-3-1, Chuo, Ami-machi, Inashikigun, Ibaraki 300-03, Japan
Yoshinori Uji
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Hiroaki Okabe
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Masataka Korenaga
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Isao Tada
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
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Abstract

The effect of linolenic acid (C18:3ω3) on the development of Strongyloides ratti first-stage larvae (L1) in culture was studied. The fatty acid composition of S. ratti free-living generations was analyzed by gas chromatography. L1 had abundant linoleic acid (C18:2ω6) but its proportion decreased with development. On the contrary, eicosapentaenoic acid (C20:5ω3) and C20:4ω3 were prominent in the filariform larva (L3). Because C20:5ω3 is generally synthesized from C18:3ω3 via C20:4ω3, the high ratio of C20:5ω3/C18:3ω3 of L3 in all the free-living generations suggested that polyunsaturated fatty acid metabolism, particularly the ω-3 series, and eicosanoids produced had important roles in the development of S. ratti L1.

Keywords

Strongyloides rattifatty acidslinolenic acidlinoleic acideicosapentaenoic acideicosanoidsdevelopment
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 4 , December 1990 , pp. 303 - 309
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Barrett, J. (1969) The effect of ageing on the metabolism of the infective larvae of Strongyloides ratti Sandground, 1925. Parasitology, 59, 3–17.CrossRefGoogle Scholar
Folch, L., Lee, M. & Stanley, G. H. S. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509.CrossRefGoogle ScholarPubMed
Frayha, G. J. & Smyth, J. D. (1983) Lipid metabolism in parasitic helminths. Advances in Parasitology, 22, 309–387.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B. & Kevin, M. B. (1985) Schistosoma mansoni: Eicosanoid production by cercariae. Experimental Parasitology, 59, 44–50.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B. & Delbrook, K. (1986) Schistosoma mansoni: Production of cercarial eicosanoids as correlates of penetration and transformation. Journal of Parasitology, 72, 397–404.CrossRefGoogle ScholarPubMed
Fusco, A. C., Salafsky, B., Whitely, K. & Yohe, S. (1987) Schistosoma mansoni: pH dependence of cercarial eicosanoid production, penetration, and transformation. Experimental Parasitology, 64, 139–146.CrossRefGoogle ScholarPubMed
Jacobsen, N. S. & Fairbairn, D. (1967) Lipid metabolism in helminth parasites. III. Biosynthesis and interconversion of fatty acids by Hymenolepis diminuta (Cestoda). Journal of Parasitology, 53, 355–361.CrossRefGoogle Scholar
Leid, R. W. & Mcconnell, L. A. (1983a) PGE2 generation and release by the larval stage of the cestode, Taenia taeniaeformis. Prostaglandins, Leukotrienes and Medicine, 11, 317–323.CrossRefGoogle ScholarPubMed
Leid, R. W. & Mcconnell, L. A. (1983b) Thromboxane A2 generation by the larval cestode, Taenia taeniaeformis. Clinical Immunology and Immunopathology, 28, 67–76.CrossRefGoogle ScholarPubMed
Liu, L. X. & Weller, P. F. (1989) Brugia malayi: Microfilarial polyunsaturated fatty acid composition and synthesis. Experimental Parasitology, 69, 198–203.CrossRefGoogle ScholarPubMed
Metcalfe, L. D. & Schmitz, A. A. (1961) The rapid preparation of fatty acid esters for gas chromatographic analysis.Analytical Chemistry, 33, 363–364.CrossRefGoogle Scholar
Meyer, F., Kimura, S. & Mueller, J. F. (1966) Lipid metabolism in the larval and adult forms of the tapeworm Spirometra mansonoides. Journal of Biological Chemistry, 241, 4224–4232.CrossRefGoogle ScholarPubMed
Minematsu, T., Mimori, T., Tanaka, M. & Tada, I. (1989) The effect of fatty acids on the developmental direction of Strongyloides ratti first-stage larvae. Journal of Helminthology, 63, 102–106.CrossRefGoogle ScholarPubMed
Salafsky, B., Wang, Y-S., Fusco, A. C. & Antonacci, J. (1984a) The role of essential fatty acids and prostaglandins in cercarial penetration (Schistosoma mansoni). Journal of Parasitology, 70, 656–660.CrossRefGoogle ScholarPubMed
Salafsky, B., Wang, Y-S, Kevin, M. B., Hill, H. & Fusco, A. C. (1984b) The role of prostaglandins in cercarial (Schistosoma mansoni) response to free fatty acids. Journal of Parasitology, 70, 584–591.CrossRefGoogle ScholarPubMed
Salafsky, B., Fusco, A. C., Whitley, K., Nowicki, D. & Ellenberger, B. (1988) Schistosoma mansoni: Analysis of cercarial transformation methods. Experimental Parasitology, 67, 116–127.CrossRefGoogle ScholarPubMed
Tada, I., Mimori, T. & Nakai, M. (1979) Migration route of Strongyloides ratti in albino rats. Japanese Journal of Parasitology, 28, 219–227.Google Scholar
Taguchi, I., Suto, C. & Kumada, N. (1989) Studies on the development and differentiation of Strongyloides ratti. I. Improved isolation method of the first-stage larvae. Japanese Journal of Parasitology, 38, 38–45. (In Japanese with English summary).Google Scholar
Turner, A. C. & Hutchison, W. F. (1979) Lipid synthesis in the adult dog heartworm, Dirofilaria immitis. Comparative Biochemistry and Physiology, 64B, 403–405.Google Scholar