Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-07T16:33:01.641Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes

Published online by Cambridge University Press:  05 June 2009

M. Larsen ,
J. Wolstrup ,
S. A. Henriksen ,
J. Grønvold  and
P. Nansen
M. Larsen
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Wolstrup
Affiliation:
Department of Ecology and Molecular Biology, Section of Microbiology, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C., Denmark
S. A. Henriksen
Affiliation:
National Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen, Denmark
J. Grønvold
Affiliation:
Department of Veterinary Microbiology, Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
P. Nansen
Affiliation:
Department of Veterinary Microbiology, Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
Get access Rights & Permissions [Opens in a new window]

Abstract

The experiment was designed to test the survival and performance of stress selected nematophagous fungi after passage through the gastro-intestinal tract of cattle. Ruminating calves were fed daily with a fixed amount of fungal material grown on barley grains. The excreted dung was collected on days four and five after the start of the feeding experiment. Barley grains were washed out of the excreted dung and incoculated on water-agar plates. After incubation for one week, nine out of ten fungal isolates were re-isolated from these plates. The predatory capacity of the fungi in the excreted faeces was tested in a dung pat bioassay and a faecal culture system. In the dung pat bioassay. two fungi of the genus Arthrobotrys and six of the genus Duddingtonia reduced the development of Ostertagia ostertagi third stage larvae by 85% (61%–93%). compared to the number of larvae developed from fungus-free control pats. In seven out of these eight isolates, the reduction of larvae in the faecal cultures was 92% (76%–99%).

Keywords

nematophagous fungiArthrobotrysDuddingtoniacattlestress selectionbiological controlOstertagia ostertagiNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 137 - 141
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Cooke, R. C. & Godfrey, B. E. S. (1964) A key to the nematode-destroying fungi. Transactions of the British Mycological Society, 47, 61–74.CrossRefGoogle Scholar
Domsch, K. H., Gams, W. & Anderson, T.-H. (1980) Arthrobotrys Corda 1839. In: Compendium of Soil Fungi, vol. 1 pp. 59–64, Academic Press.Google Scholar
Grønvold, J., Wolstrup, J., Henriksen, S. A. & Nansen, P. (1987) Field experiments on the ability of Arthrobotrys oligospora (Hyphomycetales) to reduce the number of larvae of Cooperia oncophora (Trichostrongylidae) in cow pats and surrounding grass. Journal of Helminthology, 61, 65–71.CrossRefGoogle ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S. A., Thylin, J. & Wolstrup, J. (1988) The capability of the predacious fungus Arthrobotrys oligospora (Hyphomycetales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. Journal of Helminthology, 62, 271–280.CrossRefGoogle ScholarPubMed
Grønvold, J., Henriksen, S. A., Nansen, P., Wolstrup, J. & Thylin, J. (1989) Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats. Journal of Helminthology, 63, 115–126.CrossRefGoogle Scholar
Grüner, L., Peloille, M., Sauvé, C. & Cortet, J. (1985) Parasitologie animale.—Survie et Conservation de l'activité prédatrice vis-à-vis de nématodes trichostrongylides après ingestion par des Ovins de trois hyphomycètes prédateurs. Comptes Rendus de l'Academie des Science, Paris, 300 (14), 525–528.Google Scholar
Hashmi, H. A. & Connan, R. M. (1989) Biological control of ruminant trichostrongylids by Arthrobotrys oligospora, a predacious fungus. Parasitology Today, 5, 28–30.CrossRefGoogle ScholarPubMed
Henriksen, S. Aa. & Korsholm, H. (1983) A method for culture and recovery of gastrointestinal strongyle larvae. Nordisk Veterinærmedicin, 35, 429–430.Google ScholarPubMed
Larsen, M., Wolstrup, J., Henriksen, S. Aa., Dackman, C., Grønvold, J. & Nansen, P. (1991) In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology, 65, 193–200.CrossRefGoogle ScholarPubMed
Lysek, G. & Nordbring-Hertz, B. (1981) An endogenous rhythm of trap formation in the nematophagous fungus Arthrobotrys oligospora. Planta, 152, 50–53.CrossRefGoogle ScholarPubMed
van Oorschot, C. A. N. (1985) Taxonomy of the Dactylaria complex, V.A review of Arthrobotrys and allied genera. Studies in Mycology, 26, 61–96.Google Scholar
Siegel, S. (1956) Non-parametric Statistics for the Behavioural Sciences. McGraw Hill Kogakusha Ltd Tokyo. 312 pp.Google Scholar
Soprunov, F. F. (1958) Predacious Hyphomycetes and their application in the control of pathogenic nematodes. Ashkhabad, 292 pp. (Translated from Russian by Nemchonok, S., Israel Program for Scientific Translocation Ltd., Jerusalem. 1966).Google Scholar