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In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes

Published online by Cambridge University Press:  05 June 2009

M. Larsen ,
J. Wolstrup ,
S. A. Henriksen ,
J. Grønvold  and
P. Nansen
M. Larsen
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Wolstrup
Affiliation:
Department of Ecology and Molecular Biology, Section of Microbiology, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C., Denmark
S. A. Henriksen
Affiliation:
National Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen, Denmark
J. Grønvold
Affiliation:
Department of Veterinary Microbiology, Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
P. Nansen
Affiliation:
Department of Veterinary Microbiology, Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
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Abstract

The experiment was designed to test the survival and performance of stress selected nematophagous fungi after passage through the gastro-intestinal tract of cattle. Ruminating calves were fed daily with a fixed amount of fungal material grown on barley grains. The excreted dung was collected on days four and five after the start of the feeding experiment. Barley grains were washed out of the excreted dung and incoculated on water-agar plates. After incubation for one week, nine out of ten fungal isolates were re-isolated from these plates. The predatory capacity of the fungi in the excreted faeces was tested in a dung pat bioassay and a faecal culture system. In the dung pat bioassay. two fungi of the genus Arthrobotrys and six of the genus Duddingtonia reduced the development of Ostertagia ostertagi third stage larvae by 85% (61%–93%). compared to the number of larvae developed from fungus-free control pats. In seven out of these eight isolates, the reduction of larvae in the faecal cultures was 92% (76%–99%).

Keywords

nematophagous fungiArthrobotrysDuddingtoniacattlestress selectionbiological controlOstertagia ostertagiNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 2 , June 1992 , pp. 137 - 141
Copyright
Copyright © Cambridge University Press 1992

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