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Rubella antibody measured by radial haemolysis. Characteristics and performance of a simple screening method for use in diagnostic laboratories*

Published online by Cambridge University Press:  15 May 2009

J. B. Kurtz ,
P. P. Mortimer ,
P. R. Mortimer ,
P. Morgan-Capner ,
M. S. Shafi  and
G. B. B. White
J. B. Kurtz
Affiliation:
Virology Laboratory, Churchill Hospital, Oxford 0X3 7LJ
P. P. Mortimer
Affiliation:
Central Public Health Laboratory, Colindale Avenue, London NW9 5HT
P. R. Mortimer
Affiliation:
Public Health Laboratory, Coventry and Warwickshire Hospital, Coventry CV1 4FH
P. Morgan-Capner
Affiliation:
Department of Medical Microbiology, Kings College Hospital Medical School, London SE5 8RX
M. S. Shafi
Affiliation:
Public Health Laboratory, Central Middlesex Hospital, Park Royal, London, NW 1O 7NS
G. B. B. White
Affiliation:
Public Health Laboratory, Fazakerley Hospital, Liverpool L9 7AL
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Summary

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A simple method for preparing radial haemolysis gels for rubella antibody screening is described. In use it gave clear zones of haemolysis when a standard serum was tested at dilutions down to 5·6 i.u./ml rubella antibody. In five lab oratories 8404 sera were screened by the method and the results were read by comparing zones of haemolysis with that of a standard serum diluted to contain 15 i.u./ml antibody. A zone ⋝ 15 i.u./ml, indicating immunity, was given by 7433 (88·4%) of the sera. No zone indicating susceptibility was seen with 748 (8·9%) sera. Small zones, < 15 i.u./ml standard, were given by 189 (2·2%) sera, and in only 34 cases (0·4%) did non-specific haemolysis interfere with the test readings. Further testing of the radial haemolysis negative and low positive sera by the haemagglutination inhibition test gave rise to some discrepant results which are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 84 , Issue 2 , April 1980 , pp. 213 - 222
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

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