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Factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick

Published online by Cambridge University Press:  15 May 2009

Ella M. Barnes ,
C. S. Impey  and
B. J. H. Stevens
Ella M. Barnes
Affiliation:
A. R. C. Food Research Institute, Colney Lane, NorwichNR4 7UA
C. S. Impey
Affiliation:
A. R. C. Food Research Institute, Colney Lane, NorwichNR4 7UA
B. J. H. Stevens
Affiliation:
A. R. C. Food Research Institute, Colney Lane, NorwichNR4 7UA
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Summary

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Thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. Under the conditions of the test only Bacteroides hypermegas and a Bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (VFA's) coupled with a low pH. When these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. Possible explanations for this observation are considered.

The pH value and concentration of VFA's in the caecal material were determined in chicks from 0–84 days. In vitro tests with s. typhimurium indicated that, whilst the organism would be able to multiply at the pH and concentration of VFA's found during the first few days after hatching, the rapid increase inVFA concentration during the first 21 days would make this increasingly difficult. The significance of the developing caecal flora in relation to VFA production and pH is discussed.

Because certain feed additives are known to influence the carriage of salmonellas, the sensitivity of various caecal anaerobes to these compounds was determined in vitro, generally at 1, 10 and 100 μg/ml. The additives tested included flavomycin, furazolidone, nitrovin, tetracycline, tylosin, sulphaquinoxaline, virginiamycin and zinc bacitracin. All the organisms tested were inhibited by 100 μg/ml furazolidone; none were inhibited by 500 μg/ml sulphaquinoxaline.

Changes occurring in the VFA concentration, pH value and microflora of the caeca of chicks fed for 49 days or longer on a normal starter diet or the same diet containing 10 or 100 mg/kg nitrovin have been compared. When the chicks were fed on the diet containing 100 mg/kg nitrovin, the Gram-negative non-sporing anaerobes were eliminated as a significant part of the caecal flora. However, the VFA concentration combined with a low pH in chicks from 2 weeks onwards was still sufficient to inhibit salmonella multiplication. Other possibly interrelated factors which might lead to an increased salmonella carrier rate in the nitrovintreated chickens are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 82 , Issue 2 , April 1979 , pp. 263 - 283
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Ashcraft, D. W. (1933). Effect of milk products on pH of intestinal contents of domestic fowl. Poultry Science 12, 292–8.CrossRefGoogle Scholar
Barnes, E. M. (1956). Methods for the isolation of faecal streptococci (Lancefield group D) from bacon factories. Journal of Applied Bacteriology 19, 193–203.CrossRefGoogle Scholar
Barnes, E. M. & Goldberg, H. (1962). The isolation of anaerobic Gram-negative bacteria from poultry reared with and without antibiotic supplements. Journal of Applied Bacteriology 25, 94–106.CrossRefGoogle Scholar
Barnes, E. M. & Impey, C. S. (1968). Anaerobic Gram-negative non-sporing bacteria from the caeca of poultry. Journal of Applied Bacteriology 31, 530–41.CrossRefGoogle Scholar
Barnes, E. M. & Impey, C. S. (1970). The isolation and properties of the predominant anaerobic bacteria in the caeca of chickens and turkeys. British Poultry Science 11, 467–81.CrossRefGoogle ScholarPubMed
Barnes, E. M. & Impey, C. S. (1972). Some properties of the non-sporing anaerobes from poultry caeca. Journal of Applied Bacteriology 35, 241–51.CrossRefGoogle Scholar
Barnes, E. M. & Impey, C. S. (1974). The occurrence and properties of uric acid decomposing anaerobic bacteria in the avian caecum. Journal of Applied Bacteriology 37, 393–409.CrossRefGoogle Scholar
Barnes, E. M., Impey, C. S., Stevens, B. J. H. & Peel, J. L. (1977). Streptococcus pleomorphus, sp.nov. An anaerobic streptococcus isolated mainly from the caeca of birds. Journal of General Microbiology 102, 45–53.CrossRefGoogle ScholarPubMed
Barnes, E. M., Mead, G. C., Barnum, D. A. & Harry, E. G. (1972). The intestinal flora of the chicken in the period 2–26 weeks of age, with particular reference to the anaerobic bacteria. British Poultry Science 13, 311–26.CrossRefGoogle Scholar
Barnes, E. M., Mead, G. C., Impey, C. S. & Adams, B. W. (1978). The effect of dietary bacitracin on the incidence of Streptococcus faecalis sub-species liquefaciens and related streptococci in the intestines of young chicks. British Poultry Science 19, 713–23.CrossRefGoogle Scholar
Beerens, H., Schaffner, Y., Guillaume, J. & Castel, M. M. (1963). Les bacilles anaérobies non sporulés à Gram-négatif favorisés par la bile. Annales de L'Institut Pasteur de Lille 14, 5–48.Google Scholar
Bohnhoff, M., Miller, C. P. & Martin, W. R. (1964 a). Resistance of the mouse's intestinal tract to experimental salmonella infection. I. Factors which interfere with the initiation of infection by oral inoculation. Journal of Experimental Medicine 120, 805–16.CrossRefGoogle ScholarPubMed
Bohnhoff, M., Miller, C. P. & Martin, W. R. (1964 b). Resistance of the mouse's intestinal tract to experimental salmonella infection. II. Factors responsible for its loss following streptomycin treatment. Journal of Experimental Medicine 120, 817–28.CrossRefGoogle ScholarPubMed
Brownell, J. R., Sadler, W. W. & Fanelli, M. J. (1969). Factors influencing the intestinal infection of chickens with Salmonella typhimurium. Avian Diseases 13, 804–16.CrossRefGoogle ScholarPubMed
Bryant, M. P. & Burkey, L. A. (1953). Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. Journal of Dairy Science 36, 205–17.CrossRefGoogle Scholar
Cato, E. P. & Johnson, J. L. (1976). Reinstatement of species ranks for Bacteroides fragilis B. ovatus, B. distasonis, B. thetaiotamicron and B. vulgatus. Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotamicron (Distaso) Castellani and Chalmers. International Journal of Systematic Bacteriology 26, 230–7.CrossRefGoogle Scholar
Elsden, S. R. & Gibson, Q. H. (1954). The estimation of lactic acid using ceric sulphate. Biochemical Journal 58, 154–8.CrossRefGoogle ScholarPubMed
Finegold, S. M., Miller, A. B. & Posnick, D. J. (1965). Further studies on selective media for Bacteroides and other anaerobes. Ernährungsforschung 10, 517–28.Google Scholar
Freter, R. (1974). Interactions between mechanisms controlling the intestinal microflora. American Journal of Clinical Nutrition 27, 1409–16.CrossRefGoogle ScholarPubMed
Gossling, F. & Moore, W. E. C. (1975). Gemmiger formicilis, n.gen., n.sp., an anaerobic budding bacterium from intestines. International Journal of Systematic Bacteriology 25, 202–7.CrossRefGoogle Scholar
Hirsch, A. & Grinsted, E. (1954). Methods for the growth and enumeration of anaerobic spore formers from cheese with observations on the effect of nisin. Journal of Dairy Research 21, 101–10.CrossRefGoogle Scholar
Holdeman, L. V. & Moore, W. E. C. (1974). New genus Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human faeces. International Journal of Systematic Bacteriology 24, 260–77.CrossRefGoogle Scholar
Hungate, R. E. (1950). The anaerobic mesophilic cellulolytic bacteria. Bacteriological Review 14, 1–49.CrossRefGoogle ScholarPubMed
Lev, M. & Briggs, C. A. E. (1956). The gut flora of the chick. 1. The flora of the newly hatched chick. Journal of Applied Bacteriology 19, 36–8.CrossRefGoogle Scholar
Meynell, G. C. (1963). Antibacterial mechanisms of the mouse gut. II. The role of Eh and volatile fatty acids in the normal gut. British Journal of Experimental Pathology 44, 209–19.Google ScholarPubMed
Mead, G. C. & Adams, B. W. (1975). Some observations on the caecal microflora of the chick during the first two weeks of life. British Poultry Science 16, 169–76.CrossRefGoogle ScholarPubMed
Nurmi, E. & Rantala, M. (1974). The influence of zinc bacitracin on the colonisation of Salmonella infantis in the intestine of broiler chickens. Research in Veterinary Science 17, 24–7.CrossRefGoogle ScholarPubMed
Ochi, Y., Mitsuoka, T. & Sega, T. (1964). Untersuchungen über die Darmflora des Huhnes. III. Mitteilung: Die Entwicklung der Darmflora von Küken bis zum Huhn. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, 1 Orig. 193, S. 80–95.Google Scholar
Rantala, M. (1974). Nitrovin and tetracycline: a comparison of their effect on salmonellosis in chicks. British Poultry Science 15, 299–303.CrossRefGoogle ScholarPubMed
Rantala, M. & Nurmi, E. (1973). Prevention of the growth of Salmonella infantis in chicks by the flora of the alimentary tract of chickens. British Poultry Science 14, 627–30.CrossRefGoogle ScholarPubMed
Rantala, M. & Nurmi, E. (1974). Hazards involved in the use of furazolidone for the prevention of salmonellosis in broiler chickens. Journal of Hygiene 72, 349–54.CrossRefGoogle ScholarPubMed
Royal, W. A. & Mutimer, M. D. (1972). Inhibition of Salmonella typhimurium by fowl caecal cultures. Research in Veterinary Science 13, 184–5.CrossRefGoogle ScholarPubMed
Smith, H. Williams (1965). The development of the flora of the alimentary tract in young animals. Journal of Pathology and Bacteriology 90, 495–513.CrossRefGoogle ScholarPubMed
Smith, H. Williams & Tucker, J. F. (1975). The effect of feeding diets containing permitted antibiotics on the faecal excretion of Salmonella typhimurium by experimentally infected chicks. Journal of Hygiene 75, 293–301.CrossRefGoogle Scholar
Van Der Weil-Korstanje, J.A.A. & Winkler, K. C. (1970). Medium for the differential count of the anaerobic flora in human faeces. Applied Microbiology 20, 168–9.CrossRefGoogle Scholar