Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-22T21:15:29.991Z Has data issue: false hasContentIssue false
  • English
  • Français

Bacteriophage inhibition by extracts from phage-insensitive bacteria of the genus Pseudomonas

Published online by Cambridge University Press:  15 May 2009

T. H. Mead  and
M. van den Ende
T. H. Mead
Affiliation:
The C.S.I.R. and University of Cape Town Virus Research Unit, Department of Pathology, University of Cape Town
M. van den Ende
Affiliation:
The C.S.I.R. and University of Cape Town Virus Research Unit, Department of Pathology, University of Cape Town
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. An electrophoretically homogeneous antigen preparation extracted from a strain (LII) of Pseudomonas aeruginosa by trichloracetic acid inhibits four phages active against this strain, and two phages (5 and 14) to which it is resistant but which attack a mutant strain LIII–3 bi.

2. An extract prepared in the same way from strain LIII–3 bi has no antigenic or inhibitory properties. Lysis of LIII–3 bi organisms by chloroform, however, yields a potent inhibitor active against several phages.

3. TCA extracts from immunologically distinct Ps. strains AII, BII and GII also inhibit phages 5 and 14 to which these strains are resistant.

4. The effect of a third phage (13) on Ps. LIII–3 bi is activated by the extract from strain LII which normally inhibits phages 5 and 14. Prolonged contact with very dilute solutions of the extract also causes activation of phage 5. Activation of phage 14 has not been observed.

5. The above findings are discussed in relation to current theories of phage action and phage inhibition.

Type
Research Article
Information
Journal of Hygiene , Volume 51 , Issue 1 , March 1953 , pp. 108 - 124
Copyright
Copyright © Cambridge University Press 1953

References

Andrewes, C. H. & Elford, W. N. (1933). Brit. J. exp. Path. 14, 367, 376.Google Scholar
Beumer, J. (1947). Rev. belg. Path. 18, 244.Google Scholar
Boivin, A. & Mesrobèanu, L. (1933). C.R. Soc. Biol., Paris, 112, 76.Google Scholar
Boivin, A. & Mesrobèanu, L. (1937). C.R. Soc. Biol., Paris, 125, 273.Google Scholar
Boyd, J. S. K. (1951). J. Path. Bact. 83, 445.CrossRefGoogle Scholar
Boyd, J. S. K. (1952). Symposium on the Nature of Virus Multiplication. London.Google Scholar
Burnet, F. M. (1927). Brit. J. exp. Path. 8, 121.Google Scholar
Burnet, F. M. (1929). J. Path. Bact. 32, 15.CrossRefGoogle Scholar
Burnet, F. M. (1930). J. Path. Bact. 33, 647.CrossRefGoogle Scholar
Burnet, F. M. (1934). J. Path. Bact. 38, 285.CrossRefGoogle Scholar
Burnet, F. M. & Freeman, M. (1937). Aust. J. exp. Biol. med. Sci. 15, 49.CrossRefGoogle Scholar
Don, P. A. & Van DenEnde, M. Ende, M. (1950). J. Hyg., Camb., 48, 196.CrossRefGoogle Scholar
Donald, R. (1915). Lancet, 2, 1243.CrossRefGoogle Scholar
Gladstone, G. P. & Fildes, P. (1940). Brit. J. exp. Path. 21, 162.Google Scholar
Goebel, W. F. (1950). J. exp. Med. 92, 527.CrossRefGoogle Scholar
Gough, G. A. C. & Burnet, F. M. (1934). J. Path. Bact. 38, 301.CrossRefGoogle Scholar
Gratia, A. (1936). Ann. Inst. Pasteur, 57, 657.Google Scholar
Hadley, P. (1926). Proc. Soc. exp. Biol., N.Y., 23, 443.CrossRefGoogle Scholar
Levine, P. & Frisch, A. W. (1933). Proc. Soc. exp. Biol., N.Y., 30, 393; 31, 46.Google Scholar
Lwoff, A., Siminovitch, L. & Kjeldgaard, N. (1950). C.R. Acad. Sci. 231, 190.Google Scholar
Mead, T. H. (To be published.)Google Scholar
Miles, A. A. & Misra, S. S. (1938). J. Hyg., Camb., 38, 732.Google Scholar
Miller, E. M. & Goebel, W. F. (1949). J. exp. Med. 90, 255.CrossRefGoogle Scholar
Morgan, W. T. J. (1937). Biochem. J. 31, 2003.CrossRefGoogle Scholar
Pope, C. G. & Stevens, M. F. (1939). Biochem. J. 33, 1070.CrossRefGoogle Scholar
Rakieten, M. L., Rakieten, T. L. & Doff, S. (1936). J. Bact. 32, 505.CrossRefGoogle Scholar
Sevag, M. G., Lackman, D. B. & Smolens, J. (1938). J. biol. Chem. 124, 425.CrossRefGoogle Scholar
Stamp, Lord (1947). J. gen. Microbiol. 1, 251.CrossRefGoogle Scholar
Van Den Ende, M. & Mead, T. H. (1952). S. Afr. J. clin. Sci. 3, 23.Google Scholar
Van Den Ende, M. (1952). J. Hyg., Camb., 50, 405.CrossRefGoogle Scholar
Weigle, J. J. & Delbrück, M. (1951). J. Bact. 62, 301.CrossRefGoogle Scholar
White, P. Bruce (1936). J. Path. Bact. 43, 591.CrossRefGoogle Scholar