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Antimicrobial susceptibilities and serotyping of Neisseria gonorrhoeae in southern Africa: influence of geographical source of infection

Published online by Cambridge University Press:  19 October 2009

C. A. Ison ,
N. S. Roope ,
Y. Dangor ,
F. Radebe  and
R. Ballard
C. A. Ison
Affiliation:
Department of Medical Microbiology, St Mary's Hospital Medical School, Norfolk Place, Paddington, London W2 1PG
N. S. Roope
Affiliation:
Department of Medical Microbiology, St Mary's Hospital Medical School, Norfolk Place, Paddington, London W2 1PG
Y. Dangor
Affiliation:
Emergent Pathogen Research Unit of the South African Medical Research Council, School of Pathology, University of Witwatersrand and South African Institute of Medical Research, Johannesburg, South Africa
F. Radebe
Affiliation:
Emergent Pathogen Research Unit of the South African Medical Research Council, School of Pathology, University of Witwatersrand and South African Institute of Medical Research, Johannesburg, South Africa
R. Ballard
Affiliation:
Emergent Pathogen Research Unit of the South African Medical Research Council, School of Pathology, University of Witwatersrand and South African Institute of Medical Research, Johannesburg, South Africa
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One hundred and ninety-two strains of Neisseria gonorrhoeae isolated from migrant mine-workers were tested for their susceptibility to antibiotics, auxotyped and serotyped. Of the total, 93 (48%) were acquired locally and 64 (33%) from different geographical locations. Plasmid-mediated resistance to penicillin was found in 28 (14·6%) strains and was associated predominantly with the presence of 5·0 kb penicillinase encoding plasmid (18/28, 64%). Chromosomal resistance to penicillin (MIC ≥ 1 mg/l) was detected in 14 (7·3%) strains. Resistance to tetracycline was chromosomally and not plasmid-mediated. Antibiotic resistance was encountered most commonly among strains acquired in Natal. The overall gonococcal population was sensitive to ceftriaxone, ciprofloxacin. spectinomycin and azithromycin. Nine auxotype/serovar (A/S) classes were encountered among penicillinase-producing N. gonorrhoeae (PPNG) compared to 24 A/S classes among non-PPNG strains. The most common A/S class was NR/IA-6 which accounted for 38% of PPNG and 15% of non-PPNG.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 297 - 305
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Ballard, RC, Fehler, HG, Bilgeri, YR, Duncan, MO, Koornhoj, HJ.A clinical and microbiological evaluation of the treatment of urethritis with minocycline. S Afr J Epidemiol Infect 1986; 1: 19–24.Google Scholar
2.Latif, AS.Sexually transmitted diseases in Harare, Zimbabwe. S Afr J Sex Transm Dis 1982; 2: 21–3.Google Scholar
3.Goovadia, YM, Van den Ende, J, Hoosen, AA, Kharsany, A.Susceptibility of penicillinase-producing and non-penicillinase-producing strains of Neisseria gonorrhoeae isolated in Durban, South Africa to 15 B-lactam antibiotics. Sex Transm Dis 1985; 15: 30–4.Google Scholar
4.Dangor, Y, Naidoo, S, Ballard, RC.The changing pattern of antimicrobial susceptibilities of Neisseria gonorrhoeae isolated in Johannesburg. S Afr J Epidemiol Infect 1991; 6: 34–6.Google Scholar
5.Young, H.Cultural diagnosis of gonorrhoea with modified New York City medium (MNYC). Br J Vener Dis 1978; 54: 30–40.Google ScholarPubMed
6.O'Callaghan, CH, Morris, A, Kirby, SM, Shingler, AH.Novel method for detection of Blactamases using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother 1972; 1: 283–8.Google Scholar
7.Knapp, JS, Tam, MR, Nowinski, RC, Holmes, KK, Sandstrom, EG.Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis 1984; 150: 44–8.Google Scholar
8.Copley, CG, Egglestone, SI.Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom. J Med Microbiol 1983; 16: 295–302.Google ScholarPubMed
9.Ison, CA, Gedney, J, Easmon, CSF.Chromosomal resistance of gonoeocci to antibiotics. Genitourin Med 1987; 63: 239–43.Google ScholarPubMed
10.Birnboim, HC, Doly, J.A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acid Res 1979; 7: 1513–23.Google ScholarPubMed
11.Dangor, Y, Mahloru, M, Duncan, MO, Exposto, F da L, Fehler, HG, Ballard, RC.The emergence of penicillinase-producing strains of Neisseria gonorrhoeae in southern Africa. S Afr J Sex Transm Dis 1985/1986; 5: 55–7.Google Scholar
12.World Health Organization. STD treatment strategies. WHO, Geneva 1989; WHO/VDT/89.447.Google Scholar
13.Hoosen, AA, O'Farrell, N, Coetzee, KK, van den Ende, J.Efficacy of various single-dose regimens of ceftriaxone in uncomplicated acute gonococcal urethritis in adult males. S Afr Med J 1990; 78: 187–9.Google Scholar
14.Ison, CA, Bindayna, KM, Woodford, N, Gill, MJ, Easmon, CSF.Penicillin and cephalopsorin resistance in gonococci. Genitourin Med 1990: 66: 351–6.Google ScholarPubMed
15.Perine, PL, Thornsberry, C, Schalla, W, Biddle, J, Siegel, MS, Wong, KH, Thompson, SE.Evidence of two distinct types of penieillinase-producing Neisseria gonorrhoeae. Lancet 1977; ii: 993–5.Google Scholar
16.Roberts, M, Falkow, S.Conjugal transfer of R plasmid in Neisseria gonorrhoeae. Nature 1977: 266: 630–1.Google ScholarPubMed
17.Anderson, B, Odugbemi, T, Johnson, S.Penicillinase-producing Neisseria gonorrhoeae from strains from Nigeria with Far Eastern type plasmid. Lancet 1982; i: 676.Google Scholar
18.Perine, PL, Totten, PA, Knapp, JS, Holmes, KK, Bentsi, C, Kluflo, CA.Diversity of gonococeal plasmids, auxotypes and serogroups in Ghana. Lancet 1983; i: 1051–2.Google Scholar
19.Bogaerts, J, Vandepitte, J, van Dyck, E, Vanhoof, R, Dekegel, M, Piot, P.In vitro antimicrobial sensitivity of Neisseria gonorrhoeae from Rwanda. Genitourin Med 1986; 62: 217–20.Google ScholarPubMed
20.Johnson, AP, Abeck, D, Wall, RA, Mabey, DCW, Taylor-Robinson, D.Plasmid content, auxotype and protein I serovar of gonococci isolated in The Gambia. Epidemiol Infect 1987; 99: 669–74.Google Scholar
21.Mason, PR, Gwanaura, L.Characterisation of plasmid profiles, serogroups and auxotypes of Neisseria gonorrhoeae from Harare, Zimbabwe. Genitourin Med 1988; 64: 303–7.Google ScholarPubMed
22.Odugbemi, TO, Brown, ST, Biddle, J, Johnson, S, Perkins, G, Dewitt, W, Albritton, WL.Plasmid profile, serogrouping and auxotyping of Neisseria gonorrhoeae isolates from Africa. Br J Vencr Dis 1983; 59: 41–3.Google ScholarPubMed
23.Plummer, FA, D'Costa, LJ, Nzanse, H. et al. Development of endemic penicillinase-produeing Neisseria gonorrhoeae in Kenya. In: The pathogenic neisseriae. Schoolnik, GK. ed. Washington DC: American Society of Microbiology, 1985: 101–4.Google Scholar
24.Plummer, FA, Simonesen, JN, Chubb, H et al. Epidemiologieal evidence for the development of serovar-specific immunity after gonococeal infection. J Clin Invest 1989; 83: 1472–6.Google Scholar
25.Ison, CA, Easmon, CSF.Epidemiology of penicillin resistant Neisseria gonorrhoeae. Genitourin Med 1991; 67: 307–11.Google Scholar
26.Ison, CA, Pepin, J, Roope, NS, Demba, E, Secka, O, Easmon, CSF.The dominance of a multiresistant strain of Neisseria gonorrhoeae among prostitutes and STD patients in The Gambia. Genitourin Med 1992; 68: 350–60.Google Scholar
27.Woodford, N, Bindayna, KM, Easmon, CSF, Ison, CA.Associations between serotype and susceptibility to antibiotics of Neisseria gonorrhoeae. Genitourin Med 1989: 65: 86–91.Google Scholar