This investigation was concerned with fauna above and within the litter, fermentation, and humus layers of the soil in a mature stand of red spruce (Picea rubens Sarg.). The following six microsites were designated for sampling: "open" and "closed" hollows, flats, and hummocks. "Open" and "closed" referred to the space among the tree crowns directly above the microsites. Three sampling techniques, namely core samplers, pitfalls and Tullgren funnels, were employed. Samples were collected weekly from spring to winter in 1966 and 1967 by all methods except that in 1966 Tullgren funnel samples were collectell biweekly. Collected fauna comprised five Phyla, 11 classes, 28 orders, and approximately 124 families. The transformed core sample and Tullgren funnel data were analyzed using an ANOVA (2-way classification) procedure. Transformed pitfall data required an ANOVA (2-way classification, with sampling) procedure. The core sample and Tullgren funnel methods indicated a significant difference (α = 0.10) among microsites based on total weekly (or biweekly) faunal catches in all but one case (which approached significance). In 1966 there were no significant differences (α = 0.10) among the pitfall microsites but in 1967 there were. In all cases there were significant differences (α = 0.10) in the total weekly or biweekly catches of fauna from the various microsites.