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Molecular characterization of the entomopathogenic fungi Lecanicillium spp. (Deuteromycota: Hyphomycetes) isolated from white pine weevil, Pissodes strobi (Coleoptera: Curculionidae), in British Columbia

Published online by Cambridge University Press:  02 April 2012

Isabel Leal ,
René I. Alfaro ,
Young Woon Lim  and
Harry H. Kope
Isabel Leal*
Affiliation:
Pacific Forestry Centre, Canadian Forest Service, Victoria, British Columbia, Canada V8Z 1M5
René I. Alfaro
Affiliation:
Pacific Forestry Centre, Canadian Forest Service, Victoria, British Columbia, Canada V8Z 1M5
Young Woon Lim
Affiliation:
National Institute of Biological Resources, Gyungseo-dong, Seo-gu, Incheon 404–708, Korea
Harry H. Kope
Affiliation:
Forest Practices Branch, British Columbia Ministry of Forests and Range, Victoria, British Columbia, Canada V8W 1R8
*
1Corresponding author (e-mail: [email protected]).
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Abstract

The entomopathogenic fungal genus Lecanicillium Gams and Zare includes species that are highly pathogenic to many genera of insects. Three species, Lecanicillium longisporum (Petch) Zare and W. Gams, L. muscarium (Petch) Zare and W. Gams, and L. pissodis Kope and Leal, were found to be entomopathogens of adult white pine weevils, Pissodes strobi (Peck), in coastal British Columbia. Morphological characteristics were used to identify these species, but variation in conidial shape and size made it difficult to classify some of the isolates into the correct species of Lecanicillium. To confirm the identity of these Lecanicillium species, we used molecular tools such as polymerase chain reaction – restriction fragment length polymorphism and DNA sequencing.

Résumé

Le genre de champignons entomopathogènes Lecanicillium Gams et Zare contient des espèces qui sont fortement pathogènes pour de nombreuses catégories d’insectes. Trois espèces, Lecanicillium longisporum (Petch) Zare et W. Gams, L. muscarium (Petch) Zare et W. Gams et L. pissodis Kope et Leal, sont des entomopathogènes des adultes du charançon du pin blanc, Pissodes strobi (Peck), dans la région côtière de la Colombie-Britannique. Les caractéristiques morphologiques nous ont servi à identifier ces espèces, mais la variabilité de la forme et de la taille des conidies rend difficile le rattachement de certains de ces isolats à la bonne espèce de Lecanicillium. Afin de confirmer l’identité de ces espèces de Lecanicillium, nous avons utilisé des outils moléculaires, tels que la réaction de polymérisation en chaîne – polymorphisme de la longueur des fragments de restriction et le séquençage de l’ADN.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 140 , Issue 2 , April 2008 , pp. 168 - 173
Copyright
Copyright © Entomological Society of Canada 2008

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