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Impact of polyunsaturated fatty acids on human colonic bacterial metabolism: an in vitro and in vivo study*

Published online by Cambridge University Press:  09 March 2007

Lorna Thompson  and
Robin C. Spiller
Lorna Thompson
Affiliation:
Department of Therapeutics, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH
Robin C. Spiller
Affiliation:
Department of Therapeutics, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH
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Abstract

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Dietary polyunsaturated fatty acids (PUFA) reduce colonic proliferation and exert a mild laxative effect. We have studied the effect of the highly unsaturatede icosapentaenoic acid ethyl ester (EPA-EE) on the growth and metabolism of colonic bacteria in vitro, and in vivo. For the in vitro study, growth was assessed by viable counts. Bacteroides thetaiotaomicron was significantly inhibited in anaerobic media containing EPA-EE at concentrations > 7 g/I. Escherichia coli was apparently resistant even at 100 g/I. For the in vivo study, ten healthy volunteers ingested 18 g EPA-EE/d for 7 d. Stool frequency, 24 h stool weight and whole-gut transit time were assessed together with breath H2 and 14CO2 excretion following oral ingestion of 15 g lactitol labelled with 0·18 MBq [14C]lactitol. The area under the breath-H2-time curve was significantly reduced by EPA-EE, from a control value of 690·3 (SE 94·2) ppm.h to 449·5 (SE 91·7) ppm.h. Percentage dose of 14CO2 excreted, total stool weight and whole-gut transit time were unaltered, being respectively 24 (SE 2)%, 281 (SE 66) g and 45 (SE 4) h with EPA-EE v. control values of 27 (SE 1)%, 300 (SE 89) g and 42 (SE 5) h. It is concluded that dietary supplementation with EPA-EE reduces breath H2 excretion without apparently impairing overall colonic carbohydrate fermentation. The observed reduction may reflect utilization of H2 to hydrogenate the five double bonds of EPA-EE.

Keywords

Polyunsaturated fatty acidsColonic bacteriaEicosapentaenoic acid
Type
PUFA and colonic bacterial metabolism
Information
British Journal of Nutrition , Volume 74 , Issue 5 , November 1995 , pp. 733 - 741
Copyright
Copyright © The Nutrition Society 1995

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