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The changes in body weight and plasma metabolite levels during leptin injection are caused by the reduction of food intake in sheep

Published online by Cambridge University Press:  18 August 2016

T. Tokuda ,
T. Matsui ,
J. Ito ,
S Torii  and
H. Yano
T. Tokuda
Affiliation:
Laboratory of Animal Nutrition, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
T. Matsui*
Affiliation:
Laboratory of Animal Nutrition, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
J. Ito
Affiliation:
Laboratory of Animal Nutrition, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
S Torii
Affiliation:
Laboratory of Animal Nutrition, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
H. Yano
Affiliation:
Laboratory of Animal Nutrition, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
*
‡To whom correspondence should be addressed.
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Abstract

Leptin is thought to act on the central nervous system as an important regulator of food intake and body weight in mice. To examine the actions of leptin in sheep, we injected recombinant mouse leptin into the lateral cerebral ventricle of ewes. Six ewes were intracerebroventricularly cannulated and given free access to forage and water. The ewes were injected with phosphate-buffered saline (PBS) as a vehicle for 5 days through the cannula and then with PBS + leptin for 7 days consecutively. Although food intake and body weight did not change during PBS injection, the administration of leptin decreased food intake and body weight. Plasma glucose levels decreased and plasma non-esterified fatty acid levels increased on the last few days of leptin administration. Approximately 2 weeks after normal food intake was recovered, the amount of diet was individually reduced in accordance with the recorded food intake during the leptin administration in each sheep. During food restriction, body weight and plasma metabolite concentrations were similarly changed as during the leptin-administration period. These results indicate that leptin decreases food intake in sheep. Moreover, it is likely that the changes in body weight and plasma metabolites were mainly induced by the suppression of food intake during leptin injection.

Keywords

body weightfatty acidsfood intakeglucosesheep
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 70 , Issue 2 , April 2000 , pp. 343 - 348
Copyright
Copyright © British Society of Animal Science 2000

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