Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-26T14:11:36.518Z Has data issue: false hasContentIssue false
  • English
  • Français

A note on the microencapsulation of pancreatic protease for protection against gastric digestion

Published online by Cambridge University Press:  02 September 2010

C. W. Lin  and
T. G. Lee
C. W. Lin
Affiliation:
Department of Animal Husbandry, National Taiwan University, Taipei, Taiwan 10764
T. G. Lee
Affiliation:
Department of Animal Husbandry, National Taiwan University, Taipei, Taiwan 10764
Get access

Abstract

Zeolite, cellulose acetate phthalate (CAP) and maize starch were used as the coating materials for encapsulating pancreatic protease, which was proposed as an additive to enhance the food efficiency and growth rate of weanling pigs. In this study, microcapsules were formed by the process of coacervation in liquid paraffin media, where the deposition time was in the range of 20 to 60 min, and the concentrations of CAP, zeolite and maize starch were 20, 40 and 120 g/kg respectively. Hardening by n-hexane was achieved with 15 min of stirring. Microcapsules generated by this method were 20 to 30 mesh in diameter. They were stable for at least 3 h in simulated gastric conditions (pH 3·97), but disintegrated rapidly under simulated intestinal conditions (pH 6·82).

Keywords

cellulose acetate phthalatefood additivemicroencapsulationpancreatic proteinase
Type
Research Article
Information
Animal Production , Volume 56 , Issue 3 , June 1993 , pp. 413 - 417
Copyright
Copyright © British Society of Animal Science 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anson, M. L. 1938. The estimation of pepson, trypsin, papain and cathepsin with hemoglobin. Journal of General Physiology 22: 79–87.CrossRefGoogle Scholar
Artusson, P., Edman, P., Leakso, T. and Sjoholin, I. 1984. Characterization of polyacryl starch microparticals as carriers for protein and drugs. Journal of Pharmaceutical Sciences 73: 1507–1513.CrossRefGoogle Scholar
Bajpai, P. K. 1986. Synthesis of mordenite type zeolite. Zeolite 6: 2–8.CrossRefGoogle Scholar
Beyger, J. W. and Nairn, J. G. 1986. Some factors affecting the microencapsulation of pharmaceuticals with cellulose acetate phthalate. Journal of Pharmaceutical Sciences 75: 573–578.CrossRefGoogle ScholarPubMed
Chang, T. M. S. and Poznansky, M. J. 1968. Semipermeable microcapsules coating catalase for enzyme replacement in catalasemic mice. Nature, London 218: 243–245.CrossRefGoogle Scholar
Gunha, T. J. 1977. In Swine feeding and nutrition, pp. 203–206. Academic Press, New York.Google Scholar
Kitajima, M., Kondo, A. and Yamagachi, T. 1970. Encapsulation with polymeric materials. Chemical Abstracts 73: 788221z.Google Scholar
Lin, C. W. and Chen, P. F. 1990. Microencapsulation of pancreatin and its structure. Journal of the Chinese Agricultural Chemical Society 28: 112–124.Google Scholar
Lukasheva, E. V., Aisina, R. B., Gracheva, I. I., Kazanskaya, N. F. and Berezine, I. V. 1977. Properties of xchymotrypsin enclosed in polycarbonate microcapsules evaluation of the role of diffusion hindrances. Biokhimiya 42: 2013–2019.Google Scholar
Malm, C. J., Emerson, J. and Hiatt, G. D. 1951. Cellulose acetate phthalate as an enteric coating material. Journal of the American Pharmaceutical Association 40: 520–521.CrossRefGoogle ScholarPubMed
Merkle, H. P. and Speiser, P. 1973. Preparation and evaluation of cellulose acetate phthalate coacervation microcapsules. Journal of Pharmaceutical Sciences 62: 1444–1448.CrossRefGoogle ScholarPubMed
Numpton, F. A. and Fishman, P. H. 1977. The application of natural zeolite in animal science and aquaculture. Journal of Animal Science 45: 1188–1203.CrossRefGoogle Scholar
Pond, W. G., Snook, J. T., Mcneill, D., Snyder, W. I. and Stilling, B. R. 1971. Pancreatic enzyme activities of pigs up to three weeks of age. Journal of Animal Science 33: 1270–1273.CrossRefGoogle ScholarPubMed
Randen, L., Nilson, J. and Edman, P. 1988. Coprecipitation of enzymes with water soluble starch — an alternative to freeze-drying. Journal of Pharmacy and Pharmacology 40: 763–766.CrossRefGoogle ScholarPubMed
Shields, J. R., Ekstron, K. E. and Mahan, D. C. 1983. Effect of weaning age and feeding method on digestive enzyme development in swine from birth to ten weeks. Journal of Animal Science 50: 257–265.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 1988. SAS/STAT User's Guide, Release 6.03. SAS Institute Inc., Cary, NC.Google Scholar
Takenaka, H., Kawashima, Y. and Lin, S. Y. 1980. Preparation of enteric-coated microcapsules for tableting by spray-drying technique and in vitro simulation of drug release from the tablet in GI tract. Pharmaceutical Sciences 69: 1388–1392.Google ScholarPubMed
Wilson, R. H. and Leibholz, J. 1981. Digestion in the pig between 7 and 35 d of age. 2. The digestion of dry matter and the pH of digesta in pigs given milk and soybean proteins. British Journal of Nutrition 45: 321–336.CrossRefGoogle Scholar