Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T14:21:42.971Z Has data issue: false hasContentIssue false
  • English
  • Français

Interactive System for Automatic Analysis of Human R-Banded Metaphases. 1. Interactive Procedure in Selection of Metaphase Digitized Images

Published online by Cambridge University Press:  01 August 2014

G. Fontana ,
P. Matteuzzi ,
G. Fabiani  and
A. Forabosco
G. Fontana
Affiliation:
Department of Human Anatomy, University of Modena, Italy
P. Matteuzzi
Affiliation:
National Center for Photogram Analysis, Bologna, Italy
G. Fabiani
Affiliation:
Department of Automatics, University of Bologna, Italy
A. Forabosco*
Affiliation:
Department of Human Anatomy, University of Modena, Italy
*
Istituto di Anatomia Umana Normale, Via Berengario 16, 41100 Modena, Italy

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A computer system analysing human metaphase images and finding banding patterns of R-banded chromosomes has been set up. This first paper describes the interactive procedure in selection of metaphase images, automatically digitized starting from microphotographic negatives.

Riassunto

RIASSUNTO

È stato messo a punto un sistema interattivo per l'analisi automatica di immagini di metafasi umane e per lo studio deimodellidibandeggiatura lungo i cromosomi in bande R. In questa prima parte sono presentati l'hardware e il software del sistema e descritte la fase di acquisizione dei dati da negativi microfotografici e le procedure interattive che permettono di intervenire sui dati-immagine in modo da rendere analizzabili automatica-mente il maggior numéro possibile di immagini. Gli interventi piii significativi sono il cambiamento del valore soglia del fondo, la separazione di cromosomi a contatto, la connessione di frammenti di cromosomi, la cancellazione di artefatti e la designazione di cromosomi che necessitano un trattamento particolare.

Résumé

RÉSUMÉ

Un système a été mis à point pour l'analyse automatique des métaphases humaines à partir de microphotographies de chromosomes en bandes R. Il s'agit d'un système intéractif dans lequel un opérateur peut intervenir sur les données-image des métaphases digitalisés, afin d'analyser automatiquement un bon nombre de métaphases. Dans cet article le hardware et le software du système sont décrits, ainsi que les opérations intéractives les plus importantes, notamment: désignation manuelle de la valeur du fond, séparation des chromosomes à contact, réunion de parties du même chromosome séparées par accident, effaçure des erreurs, désignation des chromosomes qui ne sont pas analysables automatiquement.

Zusammenfassung

ZUSAMMENFASSUNG

Beschreibung eines interaktiven Systems zur automatischen Analyse menschlicher Metaphasenbilder und zur Erforschung der Modelle in den R-Band-Chromosomen. In diesem ersten Teil werden hardware und software des Systems beschrieben, die Datenerhebung aus negativen Mikrophotographien und die interaktiven Verfahren, die eine automatische Analyse der meisten Bilder ermöglichen. Diese Verfahren sind: Wechsel des Hintergrundschwellenwertes, Trennung der sich berührenden Chromosome, Vereinigung von Chromosomen-Fragmenten, Beseitigung der Flecken sowie die Bestimmung der Chromosomen, die einer besonderen Behandlung bedürfen.

Type
Research Article
Information
Acta geneticae medicae et gemellologiae: twin research , Volume 24 , Issue 3-4 , 07-10 1975 , pp. 299 - 306
Copyright
Copyright © The International Society for Twin Studies 1975

References

REFERENCES

Castleman, K.R., Wall, R.J. 1973. Automatic systems for chromosome identification. In Caspersson, T. and Zech, L. (eds.):. Nobel 23, Chromosome Identification [pp. 77–84]. New York: Academic Press.Google Scholar
Dimedio, G., Fontana, G. 1974. Tecniche software di preelaborazione su rappresentazioni matri-ciali di immagini piane. Rass. di Bioing., 5: 30–37.Google Scholar
Dutrillaux, B. 1974. New techniques in the study of human chromosomes. Birth Defects, 59–70.Google Scholar
Forabosco, A., Fontana, G., Fabiani, G., Bolzani, R., Toni, G., Sarti, E. 1975. Computer analysis of reverse banded chromosomes in human mata-phases. In: Biomedical Computing. Pitmans Medical Publishing (in press).Google Scholar
Gallus, G. 1968. Analisi dei contorni nel riconosci-mento di patterns per la classificazione dei cromosomi umani. Applic. Biom. Calc. Elettr., 2: 109–118.Google Scholar
Gallus, G., Montanaro, N., Maccacaro, G.A. 1969. A problem of pattern recognition in the automatic analysis of chromosomes: locating the centromere. Comp. Biomed. Res., 2: 187–197.Google Scholar
Gallus, G., Neurath, P.W. 1970. Improved computer chromosome analysis incorporating preprocessing and boundary analysis. Phys. Med. Biol., 15: 435–445.Google Scholar
Gallus, G., Regoliosi, F. 1973. Un modello decisio-nale di riconoscimento applicato ai contorni cromosomici. Applic. Biom. Calc. Elettr., 2: 69–82.Google Scholar
Ledley, R.S. 1964. High-speed automatic analysis of biomedical pictures. Science, 146: 216–232.CrossRefGoogle ScholarPubMed
Ledley, R.S. 1969. Automatic pattern recognition for clinical medicine. Proc. IEEE, 57: 2017–2035.CrossRefGoogle Scholar
Lubs, H.A., Ledley, R.S. 1973. Automated analysis of differentially stained human chromosomes. In Caspersson, T. and Zech, L. (eds.): Nobel 23, Cromosome Identification [pp. 61–76]. New York: Academic Press.Google Scholar
Matteuzzi, P., Fontana, G., Fabiani, G., Bacilieri, P., Farabosco, A. 1975. Sistema interattivo per la digitalizzazione di immagini piane di materiale biologico. Rass. Bioing., (in press).Google Scholar
Mendelsohn, M.L., Hungherford, D.A., Mayall, B.H., Perry, B., Conxay, T., Prewitt, J.M.S. 1969. Computer oriented analysis of human chromosomes. Ann. C. Y. Acad. Sci., 157: 376–392.Google Scholar
Mendelsohn, M.L., Mayall, B.H., Prewitt, J.M.S. 1969. Approaches to the automation of chromosome analysis. Image processing in Biological Science, Univ. of Cal. Press: 119–136.CrossRefGoogle Scholar
Neurath, P.W., Ampola, M.G., Low, D.A., Selles, W.D. 1970. Combined interactive computer measurement and automatic classification of human chromosomes. Cytogenetics, 9: 424–435.CrossRefGoogle ScholarPubMed
Rutovitz, D. 1968. Automatic chromosome analysis. Brit. Med. Bull., 24: 260–266.CrossRefGoogle ScholarPubMed
Rutovitz, D., Cameron, J., Farrow, A.S., Goldberg, R., Green, D.K., Hilditch, C.J. 1970. Instrumentation and organisation for chromosome measurement and karyotype analysis. Human population cytogenetics. Pfizer Medical Monography, 6: 282–296.Google Scholar