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Chapter 12 - Oocyte Preparation for Conventional In Vitro Fertilisation and Intracytoplasmic Sperm Injection

Published online by Cambridge University Press:  09 November 2022

Rachel Cutting
Affiliation:
Human Fertilisation and Embryology Authority, London
Mostafa Metwally
Affiliation:
Sheffield Teaching Hospitals and the University of Sheffield
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Summary

The aim of conventional in vitro fertilisation (cIVF) and intracytoplasmic sperm injection (ICSI) treatment cycles is to help people with fertility problems achieve their dream of having healthy live offspring. Successful treatment involves many steps in order to ensure that the oocytes are safely collected and prepared, so that fertilisation can take place in vitro. Once two pronuclei form in the oocytes, each zygote then has the opportunity to develop into a pre-implantation embryo. The most viable embryo can then be selected for transfer to the uterus for implantation hopefully to take place.

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Publisher: Cambridge University Press
Print publication year: 2022

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References

Commission of the European Parliament. Directive 2004/23/EC of the European Parliament and of the Council of 31 March 2004 on Setting Standards of Quality and Safety for the Donation, Procurement, Testing, Processing, Preservation, Storage and Distribution of Human Tissues and Cells. Brussels: European Union, 2004. https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2004:102:0048:0058:en:PDFGoogle Scholar
Georgiou, EX, Melo, P, Brown, J, Granne, IE Follicular flushing during oocyte retrieval in assisted reproductive techniques. Cochrane Database Syst Rev 2018;4:CD004634. doi: 10.1002/14651858.CD004634.pub3Google ScholarPubMed
ESHRE Working Group on Ultrasound in ART. Recommendations for good practice in Ultrasound: oocyte pick-up. Hum Reprod Open 2019;2019(4):hoz025. doi: 10.1093/hropen/hoz025CrossRefGoogle Scholar
Leese, HJ. History of oocyte and embryo metabolism. Reprod Fertil Dev 2015;27(4):567–71. doi: 10.1071/RD14278CrossRefGoogle ScholarPubMed
Sunde, A, Brison, D, Dumoulin, J, et al. Time to take human embryo culture seriously. Hum Reprod 2016;31(10):2174–82. doi: 10.1093/humrep/dew157CrossRefGoogle ScholarPubMed
Raffo, FGE, Blaquier, J. Transport IVF-ICSI: Results of a 25-year experience. JBRA Assist Reprod 2018;22(2):123–7. doi: 10.5935/1518-0557.20180026Google ScholarPubMed
Ebner, T, Moser, M, Shebl, O, et al. Blood clots in the cumulus–oocyte complex predict poor oocyte quality and post-fertilization development. Reprod Biomed Online 2008;16(6):801–7. doi: 10.1016/s1472-6483(10)60145-9.CrossRefGoogle ScholarPubMed
Frapsauce, C, Pionneau, C, Bouley, J, et al. [Unexpected in vitro fertilisation failure in patients with normal sperm: a proteomic analysis.] Gynecol Obstet Fertil 2009;37(10):796802. doi: 10.1016/j.gyobfe.2009.07.014CrossRefGoogle ScholarPubMed
Dyer, S, Chambers, GM, de Mouzon, J, et al. International Committee for Monitoring Assisted Reproductive Technologies world report: Assisted Reproductive Technology 2008, 2009 and 2010. Hum Reprod 2016;31(7):1588–609. doi: 10.1093/humrep/dew082CrossRefGoogle ScholarPubMed
Drakopoulos, P, Garcia-Velasco, J, Bosch, E, et al. Correction to: ICSI does not offer any benefit over conventional IVF across different ovarian response categories in non-male factor infertility: a European multicenter analysis. J Assist Reprod Genet 2019;36(10):2077–8. doi: 10.1007/s10815-019-01586-8Google Scholar
Gennarelli, G, Carosso, A, Canosa, S, et al. ICSI versus conventional IVF in women aged 40 years or more and unexplained infertility: a retrospective evaluation of 685 cycles with propensity score model. J Clin Med 2019;8(10):1694. doi: 10.3390/jcm8101694CrossRefGoogle ScholarPubMed
Rienzi, L, Bariani, F, Dalla Zorza, M, et al. Comprehensive protocol of traceability during IVF: the result of a multicentre failure mode and effect analysis. Hum Reprod 2017;32(8):1612–20. doi: 10.1093/humrep/dex144CrossRefGoogle ScholarPubMed
Le Bras, A, Hesters, L, Gallot, V, et al. Shortening gametes co-incubation time improves live birth rate for couples with a history of fragmented embryos. Syst Biol Reprod Med 2017;63(5):331–7. doi: 10.1080/19396368.2017.1336581CrossRefGoogle ScholarPubMed
de Vos, A, van Landuyt, L, van Ranst, H, et al. Randomized sibling–oocyte study using recombinant human hyaluronidase versus bovine-derived Sigma hyaluronidase in ICSI patients. Hum Reprod 2008;23(8):1815–19. doi: 10.1093/humrep/den212CrossRefGoogle ScholarPubMed
Ebner, T, Moser, M, Sommergruber, M, Shebl, O, Tews, G Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development. Hum Reprod 2006;21(11):2972–7. doi: 10.1093/humrep/del272CrossRefGoogle ScholarPubMed
Montag, M, Schimming, T, van der Ven, H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online 2006;12(4):442–6. doi: 10.1016/s1472-6483(10)61996-7.Google Scholar
Dib, LA, Araújo, MC, Giorgenon, RC, et al. Noninvasive imaging of the meiotic spindle of in vivo matured oocytes from infertile women with endometriosis. Reprod Sci 2013;20(4):456–62. doi: 10.1177/1933719112459217CrossRefGoogle ScholarPubMed
Swiatecka, J, Bielawski, T, Anchim, T, et al. Oocyte zona pellucida and meiotic spindle birefringence as a biomarker of pregnancy rate outcome in IVF-ICSI treatment. Ginekol Pol 2014;85(4):264–71. doi: 10.17772/gp/1722CrossRefGoogle ScholarPubMed

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