An experiment was established at three sites to determine the longevity and distribution of rhizome buds of two quackgrass biotypes in the soil profile under two tillage systems (no-till or fall-plow). The experiments were established in May 1987 at sites initially free of quackgrass. Rhizome segments with three nodes were planted 2 to 5 cm deep to give an initial density of 21 buds m-2. Production of new buds was prevented in subsequent years. Rhizome bud populations were sampled every 6 mo between October 1987 and October 1989. Buds were produced until snow cover, indicating the need to control quackgrass until late fall to prevent a bud population increase. Under no-till conditions, 94% of quackgrass buds occurred in the top 10 cm of soil while 68 and 19% were found below 10 and 20 cm, respectively, in the fall-plow treatments. A control program against quackgrass should aim at keeping the majority of rhizomes in the top 10 cm in order to facilitate control by having most shoots emerging at the same time. There was no statistically significant difference in bud longevity between biotypes, sites, and tillage systems. Viability of the rhizome buds declined rapidly during the first year. In most cases it took 2 yr for the bud populations to reach extinction although 0.6% of buds of one biotype survived as long as 30 mo in one of the three sites. Therefore, a minimum of 2 yr of total control would be required to eradicate quackgrass from most locations.