A 2-yr experiment repeated at five locations across the northeastern United States evaluated the effect of weed density and time of glyphosate application on weed control and corn grain yield using a single postemergence (POST) application. Three weed densities, designed to reduce corn yields by 10, 25, and 50%, were established across the locations, using forage sorghum as a surrogate weed. At each weed density, a single application of glyphosate at 1.12 kg ai/ha was applied to glyphosate-resistant corn at the V2, V4, V6, and V8 growth stages. At low and medium weed densities, the V4 through V8 applications provided nearly complete weed control and yields equivalent to the weed-free treatment. Weed biomass and the potential for weed seed production from subsequent weed emergence made the V2 timing less effective. At high weed densities, the V4 followed by the V6 timing provided the most effective weed control, while maintaining corn yield. Weed competition from subsequent weed emergence in the V2 application and the duration of weed competition in the V8 timing reduced yield on average by 12 and 15%, respectively. This research shows that single POST applications can be successful but weed density and herbicide timing are key elements.