Field experiments were conducted from 1995 to 1997 to test approaches to managing atrazine-resistant Datura stramonium. Eight weed management programs in a Zea mays and Glycine max rotation were evaluated for their effects on the dynamics of atrazine-resistant and -susceptible D. stramonium populations. Overall D. stramonium density and relative abundance of resistant (R) biotypes were greatly reduced in no-till Secale cereale L. (rye) cover crop management programs without triazine compared to conventional-tillage systems with the application of triazine herbicides. The negative effects of no-till on D. stramonium were greater under a G. max–Z. mays—G. max (SCS) rotation than under a Z. mays–G. max—Z. mays (CSC) rotation. A cropping system involving more G. max phases under no-till reduced both the resistant and susceptible D. stramonium populations. Results from this study support the use of soil management, crop rotation, and negative cross-resistant herbicides to manage atrazine-resistant biotypes.