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Through-grid wicking enables high-speed cryoEM specimen preparation

Published online by Cambridge University Press:  30 July 2021

Yong Zi Tan  and
John Rubinstein
Yong Zi Tan
Affiliation:
The Hospital for Sick Children, United States
John Rubinstein
Affiliation:
The Hospital for Sick Children, Canada

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 526 - 528
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Glaeser, R.M., 2021. Preparing Better Samples for Cryo–Electron Microscopy: Biochemical Challenges Do not End with Isolation and Purification. Annual Review of Biochemistry, 90.CrossRefGoogle Scholar
Tan, Y.Z. and Rubinstein, J.L., 2020. Through-grid wicking enables high-speed cryoEM specimen preparation. Acta Crystallographica Section D: Structural Biology, 76(11).Google ScholarPubMed
Rubinstein, J.L., Guo, H., Ripstein, Z.A., Haydaroglu, A., Au, A., Yip, C.M., Di Trani, J.M., Benlekbir, S. and Kwok, T., 2019. Shake-it-off: a simple ultrasonic cryo-EM specimen-preparation device. Acta Crystallographica Section D: Structural Biology, 75(12), pp. 1063-1070.CrossRefGoogle ScholarPubMed
Guo, H., Suzuki, T. and Rubinstein, J.L., 2019. Structure of a bacterial ATP synthase. Elife, 8, p. e43128.CrossRefGoogle ScholarPubMed
Guo, H., Courbon, G.M., Bueler, S.A., Mai, J., Liu, J. and Rubinstein, J.L., 2021. Structure of mycobacterial ATP synthase bound to the tuberculosis drug bedaquiline. Nature, 589(7840), pp. 143-147.CrossRefGoogle Scholar
Noble, A.J., Wei, H., Dandey, V.P., Zhang, Z., Tan, Y.Z., Potter, C.S. and Carragher, B., 2018. Reducing effects of particle adsorption to the air–water interface in cryo-EM. Nature methods, 15(10), pp. 793-795.CrossRefGoogle Scholar
Tan, Y.Z., Baldwin, P.R., Davis, J.H., Williamson, J.R., Potter, C.S., Carragher, B. and Lyumkis, D., 2017. Addressing preferred specimen orientation in single-particle cryo-EM through tilting. Nature methods, 14(8), pp. 793-796.CrossRefGoogle ScholarPubMed