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Digital Holographic Microscopy

Published online by Cambridge University Press:  02 July 2020

Tong Zhang ,
Ichirou Yamaguchi  and
Hywel Morgan
Tong Zhang
Affiliation:
Optical Engineering Lab., The Institute of Physical and Chemical Research (RIKEN) Hirosawa, Wako, Saitama, 351-0198, Japan.
Ichirou Yamaguchi
Affiliation:
Optical Engineering Lab., The Institute of Physical and Chemical Research (RIKEN) Hirosawa, Wako, Saitama, 351-0198, Japan.
Hywel Morgan
Affiliation:
Dept. of Electronics and Electrical Engineering, Univ. of Glasgow, Glasgow, G12 8QQ, UK.
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Extract

We applied phase-shifting digital holography to microscopy in this paper. At first lensless microscopy is proposed, in which no optical adjustment is necessary. Then, the method is applied to relax the limitation of focal depth in traditional optical microscopy. A theory for image formation and experimental verification using a few specimens are described.

keywords: microscopy, digital holography, phase shifting

Due to the finite focal depth of an imaging lens, a limitation to normal optical microscopy-is that, only the 2-dimensional (2-D) information of an object can be obtained at one time. Besides, it is not convenient for quantitative analysis the observed image. Optical sectioning microscopy (OSM) and scanning confocal microscopy (SCM) which use opto-electronic detection have been proposed for quantitative analysis of a 3-D object. However, the former requires critical mechanical adjustment, while the latter uses timeconsuming mechanical 3-D scanning. Holographic microscopy can solve these problems because it can record 3-D information at one time. But, the chemical processing of holograms and the mechanical focusing at the reconstructed images cause more or less trouble. A 3-D imaging technique without use of photographic recording called optical scanning holography has recently been reported. However, there are also some trouble owing to the twin-image noise.

Type
Advances in Digital Imaging
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 362 - 363
Copyright
Copyright © Microscopy Society of America

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References

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