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Permeability Oxygen Through Endothelium By Time-Resolved Fluorescence Microscopy : Influence.of Plasma Viscosity on Tissue Hypoxia.

Published online by Cambridge University Press:  02 July 2020

Dumas Dominique ,
Latger Véronique ,
Marie L. Viriot  and
Jean F. Stoltz
Dumas Dominique
Affiliation:
Laboratoire d'Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les- Nancy, France., [email protected].
Latger Véronique
Affiliation:
Laboratoire d'Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les- Nancy, France., [email protected].
Marie L. Viriot
Affiliation:
DCPR (GRAPP) - UMR CNRS 7630, ENSIC - INPL, 1 rue Grandville, 54000, Nancy, France.
Jean F. Stoltz
Affiliation:
Laboratoire d'Angiohématologie et Hémorhéologie - UMR CNRS 7563 LEMTA et équipe FR W0070 CNRS - UHP - INPL - CHU, Faculté de Médecine, F-54505 Vandoeuvre-les- Nancy, France., [email protected].
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Extract

Vascular endothelial cells (EC) forms a continuous monolayer, a selective barrier that coats the inner surface of the vascular system, representing the primary anatomical site between blood and tissue. Under closed conditions (shear stress, hypoxia-reoxygenation..), EC are activated and the level of adhesion molecules (ICAM-1) expression increased. A technique of fluorescence quenching by oxygen in cell membrane was used to study molecular transport processes on a larger scale1-4. Collisional fluorescence quenching is described by the Stern-Volmer equation : τ0 / τ = 1 + kd.PO2 where τ0 and τ are respectively the excited state lifetimes in the absence and in the presence of oxygen, Kd the quenching dynamic constant and PO2 the oxygen partial pressure. The main purpose of the present study was to investigate the relation a) between the plasma viscosity and PO2 across human EC monolayer by Time-Resolved Fluorescence Microscopy (TRFM5-6 ) between the PO2 calculated and the level of ICAM-1 expression by flow cytometry (FCM)7 and Conventional Optical Scanning Microscopy (COSM8).

Type
Biomedical Applications
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1106 - 1107
Copyright
Copyright © Microscopy Society of America

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References

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