Thirty-five species of lichens were collected from nine locations on or adjacent to the Navajo Indian Reservation in the southwestern United States. Wool was dyed using the traditional boiling water and ammonia fermentation methods. An additional method was developed using a solvent to extract lichen substances and dye wool, cold; this dimethylsulphoxide extraction method is described and the resulting dye colours were sometimes different. Over 155 individual dye tests were made on sheep's wool; a correlation of dye colour with lichen substances reported for the species was attempted. Predominant dye colours were tan, but yellow, orange, pink, purple, and blue-grey were also produced. These colours were further altered by modifying: (1) fermentation time; (2) dyeing time, temperature, or pH level; (3) exposure to light; and (4) subsequent additional extractions using the same method or different methods. Dyed wool samples, tested for stability in sunlight, generally faded to some degree and some changed colour. Most dyes obtained through the dimethylsulphoxide extraction method were light stable.

Caloplaca thuringiaca Søchting & Stordeur is described from detritus on dry south-exposed slopes in Thuringia, central Germany. The species belongs to the Caloplaca holocarpa complex, but is different from the other terricolous taxa described in the complex, which all have alpine or polar distributions.

A simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restriction enzymes

The lichen flora of the county of Gwynedd, north-west Wales, is described. The flora includes 851 species and 19 subspecies and varieties. The work comprises sections on: climate; geology; land use and vegetation; history of lichenology, 1680–1985; corticolous flora, with classification and description of the important woodland sites; inland saxicolous flora, dealing with acid, intermediate and basic rock, the effects of altitude and aspect, the flora of seepages, streams and lakes; heath and moorland flora; coastal flora classified into the recognized zones of the marine littoral, mesic, submesic and xeric supralittoral; aspects of conservation, pollution; and the lichen flora itself. The latter includes a brief description of the ecology and distribution of each species and all of the currently known 10-km2 records for the species in the county.

During 2010–12 collecting seasons, we visited 27 islands, locally called keys, in Florida Bay within the boundaries of Everglades National Park for the purpose of investigating their lichen flora. A disproportionate number of the resultant collections belong to Enterographa Fée, a genus mostly tropical in distribution. Currently, 11 species are known from Everglades National Park, of which Enterographa bradleyana, E. caudata, E. murrayana and E. nitidula are described here as new to science. Enterographa bradleyana is superificially similar to Enterographa divergens but has smaller ascospores, a wider perispore and contains gyrophoric acid. Enterographa candata is easily identified by an unusual chemistry of lichexanthone and schizopeltic acid and its extremely long tailed ascospores. Enterographa murrayana resembles E. anguinella in the field but has a different chemistry, wider ascospores with more septation and a wider perispore. Enterographa nitidula has an unusual fine powdery and glossy thallus, small 4-celled ascospores and contains an unidentified substance. Because a large number of Enterographa have been described (20 including those newly described here) since Sparrius monographed the genus in 2004, an updated world key to the genus is provided.

Thin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.

Three names in the genus Graphis are validated: Graphisnorvestitoides Sutjaritturakan, G. rongklaensis Sutjaritturakan, and Graphis arbusculaeformis (Vain.) Lücking. In addition, the new species G. pseudoaquilonia Lücking is described, differing from G. norvestitoides in the clear versus inspersed hymenium.

Eight species of Herpothallon are recorded from the Andaman Islands, four of which are described as new: Herpothallon coralloides, H. globuliferum, H. lutescens and H. minutum. All these new species have a non-pigmented thallus, prothallus, hypothallus and pseudisidia. Herpothallon coralloides and H. lutescens possess cylindrical pseudisidia whereas H. globuliferum and H. minutum have granular to globose pseudisidia. Herpothallon globuliferum is foliicolous. Herpothallon echinatum, H. granulare, H. granulosum and H. philippinum are reported for the first time from the Andaman Islands. A key to the Indian species is provided. Heiomasia pallescens (Graphidaceae), a byssoid taxon with a disc-shaped isidioid structure, is also described as new to science.

The foliose lichens Pseudocyphellaria pilosella and P. piloselloides are characterized by a cyanobacterial photobiont, a tomentose upper surface, a yellow medulla and yellow pseudocyphellae. The latter species has long been recognized as the sorediate counterpart of the former. The morphological, anatomical, chemical, and molecular analyses performed for this study support their treatment as a single species.

Lepraria granulata Slavíková, a new species from Eastern and Central Europe, is described. According to its morphology, ITS sequence and ecology, it belongs to the L. neglecta core group. Lepraria granulata is characterized by producing atranorin and two, so far unidentified, fatty acids (‘granulata unknown 1 & 2’) and phylogenetic analyses show that it is clearly distinguished from previously known species. Two additional taxa, provisionally named Lepraria sp. G and Lepraria sp. BG, are also discussed. According to their morphology and ITS sequences, they also belong to the L. neglecta group. Lepraria sp. G is sister to L. granulata whereas Lepraria sp. BG is basal and well separated from all taxa so far known in the group. Their taxonomic status is not yet entirely clear.

Species identification in the tropical lichen genus Phyllopsora is generally challenging and is based on ascospore morphology, vegetative dispersal units, thallus structure and secondary chemistry. As several type specimens are in poor condition and difficult to interpret, it is often unclear how these old names fit with the currently used taxonomy. In the present study, we aim to identify species boundaries in Phyllopsora s. str. supported by an integrative approach using multiple sources of evidence. We investigated a substantial amount of herbarium as well as freshly collected material and generated mtSSU and ITS sequence data from most of the described species, including several types. Species delimitation analyses are applied on the gene trees using mPTP and we construct a species tree of both markers with *BEAST, facilitating discussion of species delimitation and sister-relationships. Comparing morphology, chemistry and molecular data, we found that the mPTP analyses split established species repeatedly. Based on our integrative results, we exclude nine species from the genus, resurrect one (P. melanoglauca Zahlbr.), reduce two into synonymy with other Phyllopsora species and describe five as new to science: Phyllopsora amazonica Kistenich & Timdal (which shares the secondary chemistry (atranorin and terpenoid pattern) with P. halei chemotype 1, but differs, e.g., in having smaller areolae that are attached to a thinner, white prothallus, and in having more persistently marginate and less convex apothecia), Phyllopsora concinna Kistenich & Timdal (which shares the secondary chemistry (atranorin and parvifoliellin) with P. parvifoliella and P. rappiana, but differs from both in forming larger isidia, having a white prothallus, apothecial margin paler than the disc, and longer and broader ascospores), Phyllopsora furfurella Kistenich & Timdal (which is here segregated from P. furfuracea based on having a white prothallus and in containing skyrin in the hypothecium (K+ red)), Phyllopsora isidosa Kistenich & Timdal (which differs from P. byssiseda in forming a more crustose thallus with more delicate isidia, and from P. isidiotyla in forming somewhat coarser, less branched isidia) and Phyllopsora neotinica Kistenich & Timdal (a neotropical species here segregated from the now exclusively paleotropical P. chodatinica, differing in containing an unknown xanthone (not chodatin)). Lectotypes are designated for Biatora pyrrhomelaena Tuck., Lecidea leucophyllina Nyl., L. pertexta Nyl., and P. brachyspora Müll. Arg. In total, we accept 54 species in the genus Phyllopsora.

Five new species of corticolous pyrenocarpous lichens are described from tropical and subtropical forests in the Chaco and Misiones provinces in NE Argentina: Aspidothelium submuriforme with globose, grey ascomata and ascospores mostly 7-septate with 0–2 oblique longitudinal septa, 25–28×8–10 µm; Pyrenula inspersoleucotrypa, characterized by a thallus without pseudocyphellae, aggregated ascomata, an inspersed hamathecium, and ascospores of 17–20×6·5–8·0 µm with diamond-shaped lumina; Pyrenula punctoleucotrypa, which has a thallus with pseudocyphellae, aggregated ascomata in a conical pseudostroma, with fused ostioles, not inspersed hamathecium, and ascospores of 10–12×4–5 µm with rounded lumina; Strigula muriconidiata, containing immersed pycnidia with hyaline, densely muriform, ellipsoid conidia, 90–103×32–35 µm; Trypethelium globolucidum, forming sessile pseudostromata with black and whitish parts, an inspersed hamathecium, ascospores 13–19-septate, (65–)83–97×11·5–14·5 µm, lumina rounded to lentiform and containing lichexanthone.

Peltigera canina thalli have been successfully transplanted onto soil in a garden and in flowerpots. Garden samples showed marked seasonality and achieved growth rates of 6·4 cm per year. Pot-grown samples showed variation in the growth of individual thallus lobes and established that, under different soil hydration regimes, permanently hydrated thalli could sustain considerable linear growth rates for at least 140 days.

The thermocouple psychrometer was used to determine water potential, Ψ and its components in the lichen Parmotrema tinctorum. Data suggested that using conventional pressure-volume curves to study the water relations of lichens may give anomalous results, possibly because lichens may contain appreciable amounts of intercellular water. A way of correcting pressure-volume curves to remove the effect of intercellular water is discussed. Parmotrema tinctorum had a very low osmotic potential at full turgor (c. −2.5 MPa), and a low bulk modulus of elasticit (c. 2.1 MPa). As a result, P. tinctorum lost turgor only when the relative water content dropped below 0.47. Likely benefits of this for the lichen are discussed.

The genera Hypotrachyna (Vainio) Hale and Pseudoparmelia Lynge are emended to include species previously accommodated in the genus Parmelina Hale. Pseudoparmelia endochromatica sp. nov., P. singularis sp. nov., Xanthoparmelia glomerulala sp. nov., X. karamojae sp. nov., and X. meruensis sp. nov. are described. The following combinations are proposed: Hypotraehyna afrorevoluta (Krog & Swinscow) comb, nov., H. aurulenta (Tuck.) comb, nov., H. damaziana (Zahlbr.) comb, nov., H. horrescens (Taylor) comb, nov., H. meyeri (Zahlbr.) comb, nov., H. minarum (Vainio) comb, nov., H. spathulata (Kurok.) comb, nov., H. spumosa (Asah.) comb, nov., H. swinscowii (Hale) comb, nov., Parmotrema vividum (Krog & Swinscow) comb, nov., Pseudoparmelia chlorea (Stizenb.) comb, nov., P. simplicior (Hale) comb, nov., P. usambarensis (Steiner & Zahlbr.) comb, nov., P. wallichiana (Taylor) comb, nov., Xanthoparmelia interrupta (Stizenb.) comb, nov., and X. kiboensis (Dodge) comb. nov. Xanthoparmelia cylindriloba Knox is reduced to synonymy with X. kiboensis.