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Establishment of co-infection and hybridization of Haemonchus contortus and Haemonchus placei in sheep

Published online by Cambridge University Press:  04 September 2018

M.C. Santos ,
M.R.V. Amarante  and
A.F.T. Amarante Open the ORCID record for A.F.T. Amarante [Opens in a new window]
M.C. Santos
Affiliation:
Universidade Estadual Paulista (UNESP), Departamento de Parasitologia, Instituto de Biociências; Botucatu - SP, Brazil
M.R.V. Amarante
Affiliation:
Universidade Estadual Paulista (UNESP), Departamento de Parasitologia, Instituto de Biociências; Botucatu - SP, Brazil
A.F.T. Amarante*
Affiliation:
Universidade Estadual Paulista (UNESP), Departamento de Parasitologia, Instituto de Biociências; Botucatu - SP, Brazil
*
Author for correspondence: A.F.T. Amarante, E-mail: [email protected]
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Abstract

This study aimed to evaluate the simultaneous infections of Haemonchus contortus and Haemonchus placei in sheep, as well as the production of hybrids. A parental group of lambs (n = 6) were mix-infected with 2000 infective larvae (L3) of H. placei and 2000 L3 of H. contortus. Faecal samples were taken from each of these six lambs to produce the first generation of L3 (F1-L3) in individual cultures. These F1-L3 were used to infect 12 lambs; six of them were euthanized at 42 days (Group F1-42) and six at 84 days (Group F1-84) post infection. Polymerase chain reaction (PCR) analysis, using species-specific primer pairs, was the gold standard method for identification of Haemonchus adult species and hybrids. The establishment rate of both species was similar in the parental group: 51.7% H. contortus and 48.3% H. placei. Of the 219 adult specimens from groups F1-42 and F1-84 analysed by PCR, eight (3.65%) were hybrids, 111 were H. contortus and 100 were H. placei. The morphological evaluation of the F1-L3 from the parental group showed a predominance of larvae with H. contortus size (51.5%) in comparison with H. placei (42.8%). In the second generation of L3 (F2-L3) produced by the F1-lambs, larvae with H. contortus morphology predominated, with 81.5% in the F1-42 group and 84.0% in the F1-84 group. In conclusion, an artificial mixed infection by H. contortus and H. placei was established in lambs and resulted in the production of a small number of hybrids among their offspring.

Keywords

gastrointestinal nematodehost-specificitymixed infectionsruminant
Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 6 , November 2019 , pp. 697 - 703
Copyright
Copyright © Cambridge University Press 2018 

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References

Achi, YL et al. (2003) Host specificity of Haemonchus spp. for domestic ruminants in the savanna in northern Ivory Coast. Veterinary Parasitology 116, 151–158.Google Scholar
Amarante, AFT (2011) Why is it important to correctly identify Haemonchus species? Revista Brasileira de Parasitologia Veterinária 20, 1–6.Google Scholar
Amarante, AFT et al. (1997) Host specificity of sheep and cattle nematodes in São Paulo state, Brazil. Veterinary Parasitology 73, 89–104.Google Scholar
Amarante, MRV et al. (2017) PCR primers for straightforward differentiation of Haemonchus contortus, Haemonchus placei and its hybrids. Journal of Helminthology 91, 757–761.Google Scholar
Bassetto, CC et al. (2011) Protection of calves against Haemonchus placei and Haemonchus contortus after immunization with gut membrane proteins from H. contortus. Parasite Immunology 33, 377–381.Google Scholar
Brasil, BSAF et al. (2012) Genetic diversity patterns of Haemonchus placei and Haemonchus contortus populations isolated from domestic ruminants in Brazil. International Journal of Parasitology 42, 469–479.Google Scholar
Bremner, KC (1955) Cytological studies on the specific distinctness of the ovine and bovine “strains” of the nematode Haemonchus contortus (Rudolphi) Cobb (Nematoda: Trichostrongylidae). Australian Journal of Zoology 3, 312–323.Google Scholar
Chaudhry, U et al. (2014) The presence of benzimidazole resistance mutations in Haemonchus placei from US cattle. Veterinary Parasitology 204, 411–415.Google Scholar
Chaudhry, U et al. (2015) Genetic evidence for hybridisation between Haemonchus contortus and Haemonchus placei in natural field populations and its implications for interspecies transmission of anthelmintic resistance. International Journal for Parasitology 45, 149–159.Google Scholar
Fávero, FC et al. (2016) Experimental infection of calves with Haemonchus placei and Haemonchus contortus: assessment of parasitological parameters. Veterinary Parasitology 217, 25–28.Google Scholar
Gilabert, A and Wasmuth, JD (2013) Unravelling parasitic nematode natural history using population genetics. Trends in Parasitology 29, 438–448.Google Scholar
Giudici, C et al. (1999) Changes in gastro-intestinal helminth species diversity in lambs under mixed grazing on irrigated pastures in the tropics (French West Indies). Veterinary Research 30, 573–581.Google Scholar
Hoberg, EP, Lichtenfels, JR and Gibbons, L (2004) Phylogeny for species of Haemonchus (Nematoda: Trichostrongyloidea): considerations of their evolutionary history and global biogeography among Camelidae and Pecora (Artiodactyla). Journal of Parasitology 90, 1085–1102.Google Scholar
Jabbar, A et al. (2014) Unexpected occurrence of Haemonchus placei in cattle in southern Western Australia. Infection, Genetics and Evolution 21, 252–258.Google Scholar
Jacquiet, P et al. (1998) Host range and the maintenance of Haemonchus spp. in an adverse arid climate. International Journal for Parasitology 28, 253–261.Google Scholar
Khalafalla, RE, Elseify, MA and Elbahy, NM (2011) Seasonal prevalence of gastrointestinal nematode parasites of sheep in northern region of Nile Delta, Egypt. Parasitology Research 108, 337–340.Google Scholar
Le Jambre, LF (1979) Hybridization studies of Haemonchus contortus (Rudolphi, 1803) and H. placei (Place, 1893) (Nematoda: Trichostrongylidae). International Journal for Parasitology 9, 455–463.Google Scholar
Le Jambre, LF (1981) Hybridization of Australian Haemonchus placei (Place, 1893), Haemonchus contortus cayugensis (Das e Whitlock, 1960) and Haemonchus contortus (Rudolphi, 1803) from Louisiana. International Journal for Parasitology 11, 323–330.Google Scholar
Lichtenfels, JR, Pilitt, PA and Hoberg, EP (1994) New morphological characters for identifying individual specimens of Haemonchus spp. (Nematoda: Trichostrongyloidea) and a key to species in ruminants of North America. Journal of Parasitology 80, 107–119.Google Scholar
Redman, E et al. (2008) Genetics of mating and sex determination in the parasitic nematode Haemonchus contortus. Genetics 180, 1877–1887.Google Scholar
Reiniger, RCP et al. (2017) Can Haemonchus placei-primary infected naïve lambs withstand Haemonchus contortus infections? Research in Veterinary Science 114, 136–142.Google Scholar
Riggs, NL (2001) Experimental cross-infections of Haemonchus placei (Place, 1893) in sheep and cattle. Veterinary Parasitology 94, 191–197.Google Scholar
Roberts, FHS, Newton-Turner, H and McKevett, M (1954) On the specific distinctness of the ovine and bovine ‘strains’ of Haemonchus contortus (Rudolphi) Cobb (Nematoda: Trichostronglidae). Australian Journal of Zoology 2, 275–295.Google Scholar
Santos, MC et al. (2014a) Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity. Veterinary Parasitology 203, 127–138.Google Scholar
Santos, MC et al. (2014b) Differentiation of Haemonchus placei from Haemonchus contortus by PCR and by morphometrics of adult parasites and third stage larvae. Revista Brasileira de Parasitologia Veterinária 23, 495–500.Google Scholar
Silva, MRL et al. (2015) Host-specificity and morphometrics of female Haemonchus contortus, H. placei and H. similis (Nematoda: Trichostrongylidae) in cattle and sheep from shared pastures in São Paulo State, Brazil. Journal of Helminthology 89, 302–306.Google Scholar
Ueno, H and Gonçalves, PC (1998) Manual para diagnóstico das helmintoses de ruminantes, 4th Edn. Tokyo: Japan International Cooperation Agency.Google Scholar
van Wyk, JA, Cabaret, J and Michael, LM (2004) Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology 119, 277–306.Google Scholar