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1067 – Effect Of Calreticulin On Hippocampus Of Post-traumatic Stress Disorder Rats

Published online by Cambridge University Press:  15 April 2020

H. Liu ,
F. Han  and
Y. Shi
H. Liu
Affiliation:
Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, China
F. Han
Affiliation:
Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, China
Y. Shi
Affiliation:
Department of Histology and Embryology, Institute of Pathology and Pathophysiology, Basic Medical College, China Medical University, Shenyang, China

Abstract

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Objective

To detect molecular chaperone calreticulin(CRT) expression on the hippocampus in the rat model of post-traumatic stress disorder(PTSD), and discuss the regulation of CRT on Ca2+ in hippocampus of PTSD rats, further provide the experiment basis for pathogenesis of memory anomaly in PTSD rats.

Methods

The single-prolonged stress(SPS) is one of the animal models was used to set up the rat PTSD models. Male Wistar rats were randomly divided into 1, 4, 7 days groups after exposure to SPS and a normal control group. The expression of CRT was detected by using immunohistochemistry, Western blotting and RT-PCR. The intracellular free calcium was examined by fluorescence spectrophotometer.

Results

The expression of CRT in the hippocampus obviously increased after SPS stimulation, and reached the peak at SPS 4d. The intracellular free calcium level in the hippocampus obviously increased, and reached the peak at SPS 1d, then gradually decreased.

Conclusions

PTSD caused endoplasmic reticulum stress(ERS), calcium overload, up-regulated expression of CRT, activation of unfolded protein response(UPR), which maybe result in cell apoptosis and maybe the pathogenesis basis on memory anomaly in PTSD rats.

Type
Abstract
Information
European Psychiatry , Volume 28 , Issue S1: Abstracts of the 21th European Congress of Psychiatry , 2013 , 28-E472
Copyright
Copyright © European Psychiatric Association 2013
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