The concentrations of LH and oestradiol-17β were measured in samples of jugular venous plasma collected frequently during an 8-h period from six acyclic cows between 21 and 27 days post partum. Fluctuations in oestradiol-17β concentrations were detected. Although the mean concentrations of oestradiol were similar whether ovulation occurred within 1 week of sampling or not, the amplitude of the fluctuations in oestradiol concentrations was greater in the three cows which ovulated within 1 week of the period of sampling. Increases in the concentration of LH were frequently followed by increases in the concentrations of oestradiol.

Twenty-six pregnant Holstein-Friesian × zebu heifers, arranged in pairs according to age, body weight, expected calving date and amount of zebu breeding, were allocated at random to two nutritional regimens during the last 12 weeks before calving. If was planned that the animals in treatment A should calve at a pre-calving live weight of 480 kg and those in treatment B should calve at 380 kg. The diet consisted of maize silage or elephant grass silage, soya-bean meal and minerals. During the first 21 days of the post-partum period both groups were given 6 kg concentrate per head daily. After this period, the feeding level was adjusted every 14 days according to the current milk yield. The mean total milk yields were 2132 kg and 1283 kg (P < 0·05) and the lengths of lactation were 239 days and 156 days (P < 0·01) for cows in treatments A and B, respectively. There was no statistically significant difference between treatments in the intervals from calving to first heat or calving to conception.

The last 10 years have seen a significant expansion in the scope of attempts to manipulate the growth of animals (Buttery, Lindsay and Haynes, 1986). The expansion of interest has been driven by a number of factors, both economic and theoretical. At the economic level the need to develop energetically and economically efficient strategies of animal production has been coupled with a renewed awareness of the implications for human health of excessive intakes of saturated fats. Emphasis then has switched from the maximization of weight gain as an end in itself towards a need to promote protein deposition at any given intake and, at the same time, to reduce the fat content of meat and meat products. These twin objectives might be achieved by one of three strategies: the promotion of protein deposition alone, because at any given rate of weight gain this will tend to minimize the rate of fat deposition (the so-called repartitioning effect); the reduction of fat gain (an approach that has received particularly close attention by those concerned primarily with human obesity); or ideally the simultaneous promotion of protein accretion and depression of that of fat.

A system of feeding concentrates at a flat rate was compared with a step system in which the rate was reduced every 6 weeks. Each system was evaluated at either medium or high level concentrates. There were two trials involving 40 British Friesian cows for weeks 3 to 20 of lactation. The flat rate treatments were 71 (medium) and 9·3 kg dry matter (DM) per day (high) and corresponding step allocations were 8·8, 7·1 and 5·4 kg DM per day or 11·7, 9·3 and 7·0 kg DM per day. Silage was given to appetite. In trial 1 this was lucerne silage of moderate quality (metabolizable energy (ME) = 8·9 MJ/kg DM) and in trial 2 high quality grass silage (ME = 11·1 MJ/kg DM) was used.

In the comparison between systems of concentrate allocation, food intakes, milk production and live-weight change were similar in each trial. In the comparison between medium or high levels of concentrates, silage DM intakes were similar in trial 1 (7·1 v. 7·0 kg/day) but not in trial 2 (9·8 v. 8·7 kg·day, P < 0·05), and milk yields were lower in trial 1 (19·0 v. 21·4 kg/day, P < 0·001) but not in trial 2 (24·1 v. 25·2 kg/day). Milk composition was similar for medium and high concentrate intakes in both trials. Cows given the medium level of concentrates had higher live-weight losses than those given the high level in trial 1 (–58 v. –24 kg, P < 0·01) and trial 2 (–22 v. –kg). When concentrates were given at the medium level with moderate quality silage several cows had acetonaemia. Also, this feeding combination, especially when concentrates were given at a flat rate, reduced the proportion of cows which conceived within a limited breeding period.

Milk production, reproduction, blood composition and health were studied in two groups of British Friesian cows given food either twice daily in the parlour (IPF group) or through automatic out-of- parlour food dispensers (OOPF group) in the first 14 weeks of two consecutive lactations. The numbers of cows in the IPF and OOPF groups were 28 and 29 respectively in year 1 and 40 and 35 in year 2.

There were no differences in milk production or reproduction in year 1 but in year 2 the OOPF group had significant proportional increases in the yield of fat (+0·079) and protein (+0·072) and the median time from calving to conception was reduced by 43·5 days. In the IPF group body condition score was lower than in the OOPF group in year 1 (2·27 v. 2·59; P < 0·001) and in year 2 (2·37 v. 2·57; P < 0·01); as were serum total protein (75·8 v. 77·5 g/l; P < 0·05) and (73·9 v. 75·8 g/l; P < 0·01) and plasma glucose concentration (215 v. 2·32 mmol/1; P < 0·001) (2·28 v. 2·42 mmol/l; P < 0·01). In year 2 only, serum urea was lower (5·52 v. 6·07 mmol/l; P < 0·001) and serum copper higher (131 v. 11·9 μmol/l; P < 0·001) in the IPF group; the activities of aspartate amino transferase (1·66 v. 1·62 logm i.u. per 1; P < 0·05) and of glutamate dehydrogenase (1·00 v. 0·70 logm i.u. per 1; P < 0·001) were higher, despite no significant difference in fat deposition in the liver. The only significant difference in the incidence of disease was fewer cases of mastitis in the OOPF group in the second year (21 v. 12 cases).

The similarity in concentrate intakes, together with higher concentrations of blood glucose and total protein and better condition score, indicate either a more efficient utilization of energy in the OOPF group or a lower consumption of forage in the IPF group. The increased activities of liver enzymes and the high serum copper coupled with the increased incidence of mastitis and poorer fertility may indicate the release of endotoxin following chronic lactic acidosis in the IPF group.

Cows (no. = 11) were classified from liver biopsies taken 7 to 10 days after calving as having mild (FL1, no. = 6), or moderate (FL2, no. = 5) fatty infiltration of the liver. Blood samples were collected from the jugular vein: (1) three times weekly and assayed for progesterone and oestradiol; (2) at 10-min intervals for 8-h periods on three occasions during the early post-partum period and assayed for LH; (3) every 2 h, with ovarian palpation performed every 4 h around the second or third post-partum ovulation, and assayed for LH and oestradiol.

Mean weekly concentrations of oestradiol were not significantly different between FL1 and FL2 cows. FL2 cows tended to have short (< 17 days) first cycles (3/4 FL2 v. 0/5 FL1) with significantly lower concentrations of plasma progesterone (P < 0·05). Mean basal concentrations of LH in the samples collected at 10-min intervals tended to be lower in FL2 than in FL1 cows; however, the difference was significant only in the 2nd week of sampling (P = 0·05). Mean basal concentrations of LH were significantly higher in weeks 3 and 4 than in week 2 in FL1 but not in FL2 cows (P < 0·05). The frequency and amplitude of LH pulses were not affected by week of sampling or by fat content of the liver. The total area under the profile of LH during the pre-ovulatory surge was lower in FL2 than in FL1 cows (P < 0·05) but peak plasma concentrations of pre-ovulatory oestradiol and timing of ovulation after the LH surge were similar.

In three successive experiments 32, 30 and 32 autumn-calving, single-suckled cows were subjected to individual planned weight reductions over winter on silage-based diets with various levels of concentrate supplement. At a similar level of weight loss, milk yield and calf gain were increased when protein supplements of low rumen degradability were given. On average, the inclusion of 0·75 kg fish meal in the diet improved calf gain by 0·13 kg/day. Results are discussed in terms of the effect of protein supplement on the efficiency of use of body reserves.

Thirty-two Friesian cows in early lactation were divided into four treatment groups to receive ad libitum a mixed diet consisting of silage (0·70) and grain-based concentrate (0·30). Fish meal was subsequently mixed into the diet at levels of 0, 40, 80 and 120 g/kg to provide crude protein concentration (g/kg dry matter) in the complete diets of 156, 181, 200 and 212 respectively. In the 2nd week after calving the yields of fat-corrected milk (FCM) were 28·5, 29·2, 32·0 and 34·9 kg/day for the four levels respectively; at this time, food intake was sufficient only to meet the calculated energy requirement for 15 kg FCM per day. Due to recurring problems with ketosis on the diet containing 120 g fish meal per kg, this treatment was terminated and the experiment continued for 15 weeks with the groups receiving 0, 40 and 80 g/kg fish meal supplements. During this time average yields of FCM were 23·5, 25·6 and 28-0 kg FCM per day respectively and energy intakes were calculated to be sufficient to meet the requirement for 18 kg FCM per day.

It appeared possible to increase milk yield by stimulating fat mobilization through giving undegraded protein supplements to underfed cows in early lactation. However, when an excessive mobilization occurred with a high supplement, and when the animals were yielding 15 to 20 kg FCM more than their metabolizable energy intake was calculated to sustain, some cows became ketotic.

This study involved two groups of calves, one group consisted of eight Angus × (Friesian × Hereford) calves born in the late summer and kept with their dams in the field. The other group consisted of 20 autumn-born Friesian calves kept indoors in individual stalls. Similar ethograms and techniques were used to study their behaviour up to 8 weeks of age, thus allowing comparisons to be made of the proportion of time spent in various activities, as well as changes in the sequences of behaviour with age. In general, the activity of the calves of both groups showed a similar trend in development. However, the field calves spent significantly more time in investigatory behaviour while the confined calves showed a significantly higher frequency of investigatory behaviour and indulged in more self grooming. The analysis of behavioural sequences showed few differences in focal points or direction of transitions from one behavioural pattern to another, suggesting that the integration of activities into developing motor patterns in the calf is determined ontogenetically with little modification by the environmental factors encountered in this study. However, the confined calves showed a greater mean number of transitions per hour. The findings are discussed in relation to the welfare of the confined calf.

The effects of long-term administration of bovine growth hormone (GH) on pre-pubertal mammogenesis and subsequent milk production were examined in pasture-fed dairy heifers. Purified bovine GH (specific activity 0·78 i.u. per mg) was administered daily for 21 weeks (0·6 mg GH per kg M 0·75) to one member of each of 12 sets of twins from 3·5 (range 2·5 to 4-5) months of age. GH administration resulted in a significantly smaller mammary gland compared with control heifers at slaughter at the end of treatment (717 v. 839 g; P < 005 (no. = 4)) and increased the proportion of mammary fat pad containing parenchyma (461 v. 383 g/kg; P < 0·01). Mammary parenchymal dry, fat-free tissue was increased in the GH-treated group (25·8 v. 22·5 g; P < 0·05), and fat-pad adipose tissue was reduced (323 v. 435 g; P < 005), compared with the control group. Morphometric analysis of the mammary gland revealed an increase in the volume fraction of connective tissue (P < 0·1) and a tendency toward less fat in GH-treated heifers compared with control heifers. Estimates of the composition of the whole gland confirmed that GH treatment reduced total mammary fat (372 v. 496 g; P < 0·05). At calving, udder volume was greater in the heifers treated with GH compared with the untreated group (14·8 v. 11·2 1; P < 0·05 (no. = 4)). However, there were no differences in the mean daily yields of milk or milk components between groups.

Two experiments were carried out with 12 non-pregnant, non-lactating beef cows (Simmental x Hereford cross), six in each experiment. In experiment 1, the cows were grazing stubble pasture and each cow was hand fed 750 g/day soya-bean meal. In experiment 2, the cows were grazing green pasture at the pre-blossom stage. Paraffin-coated magnesium ferrite (MF) was used as an external marker. A capsule containing 5 g marker was administered to each cow twice daily at about 08.00 and 14.00 h. At the same time the cows were also weighed and faecal grab samples (GS) were taken. Each experimental period lasted 18 days: a 10-day period for attaining steady state of the marker, followed by an 8-day period during which faecal GS were taken.

Average live weight (LW, kg) and daily faecal output (DFO, kg dry matter (DM) per day) were, respectively 364·2 and 3·39 for cows in experiment 1, and 484·4 and 4·62 in experiment 2. DFO (g): LW (kg) ratios were 9·31: 1 and 9·53: 1 in experiments 1 and 2, respectively.

In-vitro digestibilities were used for calculating the voluntary intake (kg DM per day), which was found to be 6·8 and 13·6 for cows grazing stubble and pre-blossom pastures, respectively. The relationships between LW of grazing cattle and their DFO and voluntary food intake are discussed.

A sward of perennial ryegrass was either directly ensiled at 181 g dry matter (DM) per kg fresh weight with formic acid applied at 3·6 1/t fresh material or wilted for 65 h to achieve a DM concentration of 407 g DM per kg. Physical and respiration loss were measured in the field and losses during ensiling were estimated from buried bags. The silages were offered to 58 British Friesian steers, initially 3 months of age and 101 kg live weight (LW). Ten steers were allocated to an initial slaughter group and the remainder were offered the two silages either ad libitum or at two restricted levels of feeding. These animals were slaughtered after a mean period of 112 days to estimate the increments in the components of the body.

Loss of DM in the field increased from 4·3 to 86·6 g/kg on wilting and proportionately 0·75 of this additional loss was due to respiration. The fermentation characteristics of the silages were broadly similar except that the concentration of ethanol was higher in the unwilted than in the wilted silage (57·6 v. 5·6 g/kg DM). Losses of DM during ensiling were slightly higher with the unwilted (104·4 g/kg ensiled) than with the wilted (80·1 g/kg) material. Losses of nitrogen were relatively higher and energy losses lower than was the loss of DM.

Wilting significantly depressed the digestibility of gross energy from 0·651 to 0·631 (P < 0·001) and at the ad libitum level steers ate proportionately 006 more wilted silage (P > 0·05) resulting in similar intakes of digestible energy. At the ad libitum level of feeding steers given wilted silage gained more LW (+55 g/day) and empty-body weight (+64 g/day) than those given unwilted silage (P < 0·05). However, steers given wilted silage contained less fat and energy in the empty body at slaughter than those given unwilted silage. Thus, wilting did not influence the efficiency of utilization of digestible energy for energy retention nor the efficiency of utilization of dietary protein for protein gain. Since efficiencies by the animal were similar and also the increased field loss on wilting was partly compensated for by a reduced silo loss, it is concluded that the proportion of energy and protein in the standing crop retained by the steers is unaffected by wilting (65 and 64 kJ/MJ and 125 and 138 g/kg N for unwilted and wilted systems respectively). Further work is needed to establish the effect of wilting for a shorter period than in the current trial by increasing the drying rate of herbage.

Five groups of Hereford × Friesian steers (initial weight 340 kg) were given either a diet of rolled barley and ammonia-treated barley straw (AS) in the ratio 60: 40 ad libitum (ASB) to support continuous growth, or were offered over a 143-day period diets of either (i) AS alone (diet AS) (ii) untreated straw (US) plus cassava (diet USC) (iii) US plus grass silage (diet USS) or US plus fish meal (0·27 kg fish meal per day) (diet USF). Both the AS and US were offered ad libitum and allowances of cassava and silage adjusted to allow the USC and USS steers to match the growth of AS steers. Steers i n the restricted groups (AS, USC, USS and USF) were then given freshly cut grass ad libitum until they reached slaughter weight; some steers required additional hay and barley to reach slaughter weight. Steers in group ASB consumed 7·4 kg dry matter (DM), gained 1·16 kg live weight per day taking 112 days to achieve the 455 kg slaughter weight. AS, USC and USS animals maintained body weight over the restricted period, whereas the USF group tended to lose weight and daily DM intakes were 4·63 kg AS (AS steers); 3·81 kg US plus 0·95 kg cassava (USC steers); 0·86 kg US plus 3·31 kg silage (USS steers); 3·5 kg US plus 0·27 kg fish meal (USF steers). When grass or grass followed by barley plus hay was given the live-weight gains of the previously restricted cattle did not differ (mean 1·0 (s.e. 0·10) kg/day). AS provided proportionately 0·30 of the metabolizable energy used to produce growth rates in excess of 10 kg/day or if given as the sole food maintained cattle without supplementation.

Plasma progesterone concentrations were measured over two collection periods from two groups of mature non-pregnant cows involved in a twin-breeding experiment. One group (T) consisted of cows obtained from private herds after having produced at least two sets of twins each; the other was a control group of similar ages and breeds. Yearling heifers from the same breeding experiment were also included. The mean progesterone concentration from cows in the T group was proportionally 0·83 of the value in control cows and the cow group effects were consistent in direction for samples obtained after single or double ovulations. Results were also consistent in early oestrous cycle (days 3 to 7, oestrus = day 0), mid cycle (days 8 to 13) but not late cycle (days 14 to 18). Concentrations were significantly higher in yearling heifers than in control cows. There was a trend for higher progesterone concentrations after double than after single ovulations assessed by rectal palpation, but differences were not significant.

Sixty-four (48 female and 16 male) spring-born Hereford × Friesian calves were used to examine the effects of sex type, heifer slaughter age and implantation with anabolic agents on growth and carcass composition. Non-implanted and implanted heifers were slaughtered after 491, 612 and 731 days and non-implanted and implanted steers (castrated on day 81) were slaughtered after 731 days. Implantation was with 36 mg resorcylic acid lactone (RAL) on days 81 and 171 and RAL plus 300 mg trenbolone acetate on days 395, 491, 583 and 658.

Carcass weights of non-implanted and implanted heifers slaughtered at 491, 612 and 731 days were 189 and 206, 218 and 235 and 290 and 326 (s.e.d. 10·1) kg respectively. Carcass lean, bone and fat weights and fat proportion increased with increasing slaughter age but proportions of lean and bone decreased.

There was no difference between heifers and steers in growth rate to 395 days but from 395 to 731 days the steers grew faster than the heifers. Non-implanted and implanted steer carcass weights at 731 days were 297 and 355 (s.e.d. 9·5) kg respectively. Steer carcasses had higher lean and bone proportions and a lower fat proportion than heifer carcasses. Implanted animals had heavier carcasses than non-implanted animals and all of the extra carcass weight consisted of lean and bone. The results indicate that non-implanted and implanted heifers and steers had similar lean proportions when the heifers were 31 and 70 kg carcass weight respectively lighter than the steers.

The effects of winter shearing on the food intake and performance of housed ewes was studied in a series of three experiments. Winter shearing resulted in a proportional increase of dry-matter intake in ewes of 0·16 and 0·43 on two silage-based diets, of 0·09 on swede-based diets, but of only 0·02 on a hay-based diet. Shearing increased intake by a greater amount in smaller ewes and a similar trend occurred on diets containing a greater proportion of barley. Shearing increased average lamb birth weight by about 600 g (4·65 kg v. 4·06 kg), apparently as an effect of shearing per se rather than as a result of the increased energy intake. An increase in gestation length of around 1·8 days accounted for one-fifth of this increased birth weight. The effect on gestation length of shearing was to increase the unnaturally short gestation of housed unshorn ewes, this short gestation being attributed to problems of heat stress in late pregnancy. Results indicated management advantages of winter shearing in favourable climatic environments.

Rumen fluid was obtained from rumen fistulated cows (two of high (HS) and two of low (LS) susceptibility to bloat) which were bred in experimental herds subjected to long-term divergent genetic selection for pasture bloat susceptibility. In vitro fermentation using strained rumen fluid from each cow with various pasture juice substrates showed no differences between HS and LS in gas production per unit of rumen fluid or in foam production per unit of gas production. Fluid from cows on grazed pasture produced greatest gas volumes. Clover juices produced more foam of similar stability to ryegrass and mixed pasture juices. Foam production per unit of gas was greater for all juices when the rumen fluid was from cows grazing bloat potent pasture.

An experiment was carried out to examine the performance of young steers given basal diets of either silage or ammoniated hay ad libitum, with or without a daily supplement of fish meal, equivalent to 0·75 g/kg live weight, over 16 weeks. Serial slaughter of animals was carried out to examine changes in body composition during the winter feeding period and the subsequent grazing season.

There was no difference in the intakes of hay and silage organic matter over the 16 weeks of treatment. Despite the increased intake of nitrogen on the ammoniated-hay diet there was no increase in the rate of protein gain. By turn-out the steers given silage had higher empty body and carcass weights, and greater contents of fat and energy. The increased rates of empty body-weight, carcass, protein, water and energy gains achieved when the supplement was given were similar for both basal diets. There was no effect of winter feeding treatment on changes in body composition during the grazing period, so that at the end of the summer period the silage-fed animals still had heavier empty body and carcass weights. Attention is also drawn to the possible dangers of feeding hay treated with anhydrous ammonia at high temperatures to such cattle.

Groups of eight Friesian steers were given one of eight diets from 114 to 300 kg live weight. The iso-energetic diets were formulated to supply four levels of protein degradability within two concentrations of crude protein (CP), giving a range of rumen-degradable protein (RDP) and undegradable dietary protein (UDP) concentrations both above and below Agricultural Research Council (1980) recommendations. There were significant (P < 0·05), positive, linear responses of mean daily dry-matter (DM) intake (DMI) (g/kg M°75) to RDP concentration (g/kg DM) for both the low CP diets (DMI = -98·0 + 1·76 (RDP); P = 0·013) and the high CP diets (DMI = -157·5 + 218 (RDP; P = 0017). For the high CP diets, there was a significant (P = 0·045) positive, linear response of live-weight gain (LWG) (kg/day) to UDP concentration (LWG = 0·47 + 0·017 (UDP); P = 0·045). No such response was observed for the low CP diets, where the range of UDP concentrations supplied was smaller than expected. For both the low and high CP diets, LWG decreased as metabolizable energy, UDP and RDP intake increased. These negative responses to nutrient intake were reflected in a wide discrepancy between observed rates of gain and those predicted by current energy and protein nutrition systems. Interactions between food intake and digestive processes, which obstruct effective interpretation of these results, should form an explicit part of any revised protein nutrition scheme.

A study of the factors affecting weight in traditionally managed goats and sheep in central Mali was undertaken during 1978–84. Sheep were heavier than goats at all ages. Birth weights of goats (2·2 kg) were significantly affected by year and season of birth, parity, type of birth, sex and system (rain-fed millet or irrigated rice). Lamb birth weights (2·9 kg) were affected by all these sources of variation except year. System did not exert a significant influence on kid weights at 30 (4·3 kg), 90 (7·5 kg) or 150 days (10·5 kg) but the random effects of flocks and dams and the fixed effects of season, year, parity, type of birth, sex and the interaction of season × year were all significant at all three ages except for year at 30 days. Lamb weights (5·7, 11·1 and 15·5 kg) were significantly affected at all three ages by all sources of variation except season × year effects at 90 and 150 days. Repeatabilities of lamb weights were higher than of kids, probably due to milk being taken for human consumption from goats. There were few significant effects at post weaning to 730 days except those related to sex. Post-partum weights of goats (26·0 kg) were not affected by system, by the type of parturition nor by the sex of the young but were affected by season and year of parturition and by parity. Ewe post-partum weights (30·6 kg) were affected only by system and parity. Changes in weight over the year, pooled for 7 years, in specific age and sex groups averaged proportionately about 005 above and below the annual mean. Weights of mature animals were about 0·05 lower in 1984 than in 1978. Seasonal and long-term effects on weight are much less marked in small ruminants than in cattle under the same management system in the central Mali environment and the allocation of future research effort should take into consideration the relative advantages of the two types of stock.